DNA extracts of several rosy-mutation-bearing strains were associated with large insertions and deletions in a defined region of the molecular map believed to include the rosy locus DNA. Large-scale, intragenic mapping experiments were carried out that localized these mutations within the boundaries of the previously defined rosy locus structural element. Molecular characterization of the wild-...

Semisynthetic protein-DNA hybrid molecules have recently attracted much attention as valuable tools for bioanalytical chemistry and nanobiotechnology. Here we describe a synthetic method for conjugating oligonucleotides to the N-terminus of recombinant proteins. Our strategy involves the conversion of amine-terminated oligonucleotides to thioester-functionalized oligonucleotides by using a bifu...

We constructed a hybrid Moloney murine leukaemia virus (MoMLV) bearing both a chimeric CD4 and the wild-type MoMLV envelope protein (Env) on its surface. The chimeric molecule, consisting of a surface domain of CD4 and the C-terminal two-thirds of MLV Env, was expressed on the cell surface. When expressed in MoMLV-infected cells, the CD4-Env chimera was incorporated into the virion as efficient...

In this paper, the effectiveness of stiffness and damping of bearing is investigated on the natural frequencies of the rotor-bearing system. The rotor-bearing system consists of a shaft, two bearings and a disk between two bearings. Parallel spring-damper in horizental and vertical directions is considered for modeling the stiffness and damping of bearings. The gyroscopic effect is also conside...

A kind of distributed hybrid thrust bearing with tilting pad is proposed in this paper, and the influence plate tilt on performance analyzed. The film thickness equation constructed, considering machining error installation synthetically which are main causes tilt. Substituting to classical calculation bearing, time-varying stiffness obtained. angle analyzed through case controlled by change pi...

Squeeze Film Dampers (SFD) are commonly used for passive vibration control of rotor-bearing systems. The Magnetorheological (MR) and Electrorheological (ER) fluids in SFDs give a varying damping characteristic to the bearing that can provide active control schemes for the rotor-bearing system. A common way to model an MR bearing is implementing the Bingham plastic model. Adding this model to th...

Some somatic hybrid cells prepared by cell fusion of MC-induced primary tumor cells with 8-azaguanine resistant L cells were immunogenic in the induction of specific resistance in syngeneic normal mice to the challenge of parental tumor cells. However, inoculations of immunogenic hybrid cells in tumor-bearing hosts evoked enhancement of tumor growth. In these mice with enhanced tumors, the leve...

Small interfering RNA (siRNA) is effective in silencing critical molecular pathways in cancer. The use of this tool as a treatment modality is limited by lack of an intelligent carrier system to enhance the preferential delivery of this molecule to specific targets in vivo. In the present study, the in vivo behavior of novel anti-NTSR1-mAb-functionalized antimutant K-ras siRNA-loaded hybrid nan...

Multiple hypothesis testing and clustering have been the subject of extensive research in high-dimensional inference, yet these problems usually have been treated separately. By defining true clusters in terms of shared parameter values, we could improve the sensitivity of individual tests, because more data bearing on the same parameter values are available. We develop and evaluate a hybrid me...

Remaining useful life (RUL) estimation is considered as one of the most central points in the prognostics and health management (PHM). The present paper describes a nonlinear hybrid ABC–MARS-based model for the prediction of the remaining useful life of aircraft engines. Indeed, it is well-known that an accurate RUL estimation allows failure prevention in a more controllable way so that the eff...