A new test system for the detection of plant growth regulating activities was successfully employed. In a screening for inhibitors of the gibberellic acid controlled synthesis of hydrolytic enzymes in embryoless wheat seeds (Triticum aestivum) 160 cultures of ascomycetes and basi­ diomycetes were tested. In the extracts of two cultures inhibitory activities were detected. From fermentations of ...

Ouabain inhibited 86RbCl uptake by 80% in rabbit gastric superficial epithelial cells (SEC), revealing the presence of a functional Na+,K+-ATPase [(Na+ + K+)-transporting ATPase] pump. Intact SEC were used to study the ouabain-sensitive Na+,K+-ATPase and K+-pNPPase (K+-stimulated p-nitrophenyl phosphatase) activities before and after lysis. Intact SEC showed no Na+,K+-ATPase and insignificant M...

The effect of Indigenous microorganisms (IMO’s) on the native soil was investigated in the present study. Supplementation of IMO’s suspension to the soil alters the physico-chemical, biological and enzyme properties of the soil. These alternations include decreases in P from 7.2 to 6.8, increase in electrical conductivity 0.36 to 1.21 (μmohs/cm), water holding capacity 0.36 to 2.2ml/g of soil o...

Hydrolytic enzymes play important roles as biocatalysts in chemical synthesis. The chemical versatility and structurally sturdy features of Candida antarctica lipase B has placed this enzyme as a common utensil in the synthetic tool-box. In addition to catalyzing acyl transfer reactions, a number of promiscuous activities have been described recently. Some of these new enzyme activities have be...

The interactions between films of cellulose and cellulase enzymes were monitored using a quartz crystal microbalance (QCM). Real-time measurements of the coupled contributions of enzyme binding and hydrolytic reactions were fitted to a kinetic model that described closely significant cellulase activities. The proposed model combines simple Boltzmann sigmoidal and 1 - exp expressions. The obtain...

The effect of 2.5% O(2) atmosphere with and without ethylene on the activities of hydrolytic enzymes associated with cell walls, and total protein profile during ripening of avocado fruits (Persea americana Mill., cv Hass) were investigated. The low 2.5% O(2) atmosphere prevented the rise in the activities of cellulase, polygalacturonase, and acid phosphatase in avocado fruits whose ripening wa...

We examined the activities of peptidases in plasma and tissues of the New Zealand Black (NZB) mouse as an animal model of human systemic lupus erythematosus, and also in serum from patients with rheumatoid arthritis and systemic lupus erythematosus. Activities of dipeptidyl peptidase II (DAP II) and post-proline cleaving enzyme (PPCE) were increased, and dipeptidyl peptidase IV (DAP IV) activit...

Abstract Unless one embraces activities as foundational, understanding in mechanisms requires an account of the means by which entities biological engage their activities—an that does not merely explain terms more basic and activities. Recent research on molecular motors (myosin kinesin) exemplifies such account, explains free energy constraints. After describing characteristic “stepping” these...