In the last few years, spring frosts have become more of a rule than an exception. There are several prevention measures available for growers and numerous being tested; however, fruitlets often affected to some extent. One common phenomena is development frost rings. The aim our study was evaluate how occurrence rings affects sugar, organic acid, phenol content in flesh peel cv. ‘Gala’ apples....

Phenolic compounds were measured in leaf tissues of apple cvs Jonagold and Golden Delicious, healthy and infected by Venturia inaequalis. Leaves were sampled from May to September 2005 and analyzed by high performance liquid chromatography. Hydroxycinnamic acids detected were chlorogenic, caffeic, ferulic and pcoumaric. In addition, the presence was ascertained of the dihydrochalcone phloridzin...

A simple, rapid, and sensitive method for the detection of posaconazole using dispersive liquid-liquid microextraction (DLLME) coupled to surface-assisted laser desorption/ionization mass spectrometric detection (SALDI/MS) was developed. After the DLLME, posaconazole was detected using SALDI/MS with colloidal gold and α-cyano-4-hydroxycinnamic acid (CHCA) as the co-matrix. Under optimal extract...

p-Nitrophenyl acetate is the most commonly used substrate for detecting the catalytic activity of esterases, including those that activate prodrugs in human cells. This substrate is unstable in aqueous solution, limiting its utility. Here, a stable chromogenic substrate for esterases is produced by the structural isolation of an acetyl ester and p-nitroaniline group using a trimethyl lock moiet...

In the present study, the reaction between 1-hydroxyethyl radicals (1-HER) and various wine-related phenolics and thiols, including gallic acid, caffeic acid, ferulic acid, 3-mercaptohexan-1-ol (3MH), cysteine (Cys), and glutathione (GSH), was studied using competitive spin trapping with electron paramagnetic resonance (EPR) and mass spectrometry. Previous studies have reported several importa...

Matrix-Assisted Laser Desorption/Ionization time-of-flight (MALDI-TOF) mass spectrometry is evaluated as an elucidation tool for structural features and molecular weights estimation of some extracted herbaceous lignins. Optimization of analysis conditions, using a typical organic matrix, namely α-cyano-4-hydroxycinnamic acid (CHCA), in combination with α-cyclodextrin, allows efficient ionizatio...

Abstract Electron‐rich phenolic substrates can be derived from the depolymerisation of lignin feedstocks. Direct biotransformations hydroxycinnamic acid monomers obtained exploited to produce high‐value chemicals, such as α‐amino acids, however reaction is often hampered by chemical autooxidation in alkaline or harsh media. Regioselective O ‐methyltransferases (OMTs) are ubiquitous enzymes natu...