When analyzing large complex protein biopharmaceuticals, ion-pairing agents imparting low pH are widely used as mobile phase additives to improve the chromatographic performance. However, one of most effective in RPLC and HILIC, trifluoroacetic acid (TFA), is known a strong suppressor MS signal limits its use hyphenated techniques. In this study, we evaluated wide range acidic find alternatives...

This review covers advances in analytical technologies for high-throughput (HTP) glycomics. Our focus is on structural studies of glycoprotein glycosylation to support biopharmaceutical realization and the discovery of glycan biomarkers for human disease. For biopharmaceuticals, there is increasing use of glycomics in Quality by Design studies to help optimize glycan profiles of drugs with a vi...

Synthetic biological approaches, such as site-directed biosynthesis, have contributed to the expansion of the chemical space of natural products, making possible the biosynthesis of unnatural metabolites that otherwise would be difficult to access. Such methods may allow the incorporation of fluorine, an atom rarely found in nature, into complex secondary metabolites. Organofluorine compounds a...

Only 2-5% of seminal fluid is composed of spermatozoa, while the rest is seminal plasma. The seminal plasma is a rich cocktail of organic and inorganic compounds including hormones, serving as a source of nutrients for sperm development and maturation, protecting them from infection and enabling them to overcome the immunological and chemical environment of the female reproductive tract. In thi...

Malmström, David 2011. Nanoparticles in Capillary Electrophoresis hyphenated with Mass Spectrometry – What are the benefits? Department of Physical and Analytical chemistry. 38 pp. Uppsala. Capillary electrophoresis is a technique that separate compounds based on charge and size. During the last two decades capillary electrophoresis (CE) and mass spectrometry (MS) has gained in interest, become...