We investigated the ability of two overlapping fragments of staphylococcal enterotoxin B (SEB), which encompass the whole toxin, to induce protection and also examined if passive transfer of chicken anti-SEB antibodies raised against the holotoxin could protect rhesus monkeys against aerosolized SEB. Although both fragments of SEB were highly immunogenic, the fragments failed to protect mice wh...

Ovarian cancer (OVCA) mainly disseminates in the peritoneal cavity. Immune functions are important to prevent OVCA progression and recurrence. The mechanism of immunosuppression, a hallmark of tumor progression, is not well understood. The goal of this study was to determine the immune system's responses and its suppression during OVCA development and progression in hens. Frequencies of CD8+ T ...

The importance of yolk antibodies (IgY) for their use in the diagnosis and treatment human diseases is shown. structural features IgY compared to immunoglobulins were studied. disadvantages obtaining hyperimmune sera from mammalian animals revealed. A feature its resistance trypsin chymotrypsin, which can contribute more effective diseases. Data on optimization technology various bird species a...

1. dept. of biology, science and research branch, islamic azad university, tehran, iran 2. national science foundation (insf), tehran, iran 3. dept. of genetics, tehran medical sciences branch, islamic azad university, tehran, iran                                   corresponding author: mrhrdad hashemi, phd; associate professor of molecular genetics tel: (+98) 2122006664 email: mhashemi@iautmu....

An indirect sandwich ELISA that can detect as little as 8 ng of African horsesickness virus (AHSV) was developed. Viral antigen was captured from suspension using an immobilized monoclonal antibody specific for an epitope on VP7, a protein that is a major constituent of the virus core. Egg-yolk derived chicken IgY directed against AHSV (serotype 3) was used as the secondary antibody. Since IgY ...

White leghorn hens were immunized intramuscularly with formalin killed Klebsiella pneumoniae cells to generate Klebsiella pneumoniae antibodies (IgY) in egg yolk. Booster injections of increasing concentrations of antigen were given to raise the antibody level in egg yolk. Amount of protein varied from 0.87 7.4mg/ml in egg yolk throughout the immunization period. The antibodies were partially p...

Methods: Four treatment groups of 12 newly weaned pigs (approximately 22 days old) were fed either a control diet (Groups 1 and 2) or the same diet containing chicken egg-yolk antibodies (IgY) at inclusion rates of 0.32% (Group 3) and 3.2% (Group 4). Pigs in Groups 2, 3, and 4 were challenged with 5 mL of a suspension containing 1011 colony forming units per mL of a K88+ isolate of Escherichia ...

Fc receptors are key players of the immune system that link the fine specificity of immunoglobulins and innate effector responses. Here, we describe a nonmammalian Fcgamma receptor, CHIR-AB1, a member of the leukocyte receptor complex, that binds IgY with high affinity with its single Ig domain. It is expressed on immature and mature B lymphocytes, monocytes, macrophages, and natural killer cel...