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BACKGROUND Optical Coherence Tomography (OCT) imaging is being increasingly used in clinical practice for the monitoring of papilloedema. The aim is to characterise the extent and location of the Retinal Nerve Fibre Layer (RNFL) Thickness automated segmentation error (SegE) by manual refinement, in a cohort of Idiopathic Intracranial Hypertension (IIH) patients with papilloedema and compare thi...
There is a vast difference in the traditional presentation of AFM data and confocal data. AFM data are presented as surface contours while confocal data are usually visualized using either surface- or volume-rendering techniques. Finding a common meaningful visualization platform is not an easy task. AFM and CLSM technologies are complementary and are more frequently being used to image common ...
Summary Transmembrane signalling plays important physiological roles, with G protein-coupled cell surface receptors being particularly important therapeutic targets. Fluorescent proteins are widely used to study signalling, but analyses of image time series can be challenging, in particular when cells change shape. QuimP software semi-automatically tracks spatio-temporal patterns of fluorescenc...
In this paper, we propose a new roundness parameter R, to denote circularity corrected by aspect ratio. The basic concept of this new roundness parameter is given by the following equation: R = Circularity + (Circularity perfect circle Circularity aspect ratio) where Circularityperfect circle is the maximum value of circularity and Circularityaspect ratio is the circularity when only the aspect...
This study uses Cartesian plane geometry to model mitotic cells and describe mathematical methods based on this quantify chromosome misalignment measure kinetochore position metaphase plates. The method is adapted count lagging kinetochores in anaphase provide semiautomated, user-friendly ImageJ macros facilitate the operations.
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