Immature avian sympathetic neurons are able to proliferate in culture for a limited number of divisions albeit expressing several neuron-specific properties. The effect of avian retroviral transfer of oncogenes on proliferation and differentiation of sympathetic neurons was investigated. Primary cultures of 6-d-old quail sympathetic ganglia, consisting of 90% neuronal cells, were infected by My...

The slaughtered cows or heifers have a large number of immature oocytes within primordial follicles or developing follicles. Therefore, it is useful to aspirate immature oocytes, which would be wasted if left unprocessed, from ovaries; subsequently they are matured in vitro (IVM), fertilized in vitro (IVF), and cultured in vitro (IVC). Also most recently it has been shown that immature oocytes ...

Tef [Eragrostis tef (Zucc.) Trotter] is the major cereal crop in the Horn of Africa particularly in Ethiopia where it is staple food for about 50 million people. Its resilience to extreme environmental conditions and high in nutrition makes tef the preferred crop among both farmers and consumers. The efficiency of in vitro regeneration plays significant role in the improvement of crops. We inve...

AimsTo evaluate whether melatonin (MT) supplementation during in vitro maturation (IVM) of human oocytes can reverse the age-related decline oocyte quality.Main methodsWe enrolled 172 patients aged ≥35 years (older reproductive-aged women) and 83 <35 (young who underwent fertilization between 2019 2022. We conducted IVM with without 10 μM MT immature different ages. Oocyte embryo development we...

Leukaemia inhibitory factor (LIF) plays an essential role during cell differentiation and implantation in the mouse exerting its effects via the two dimerizing receptor subunits glycoprotein 130 (gp130) and LIF-receptor beta (LR-beta). This study investigated the mRNA expression of bovine LIF (bLIF), gp130 and LR-beta in pooled or individual bovine oocytes and embryos generated in vitro or in v...

Embryo rescue refers to an in vitro technology have been used for propagating plants under from poorly developed embryos by avoiding embryo abortion. The aim of the present study was regeneration a medicinally wild tree species Buchanania lanzan through method. Isolated immature dissected out de-coded seeds collected 15 years old trees were cultured Woody Plant Medium (WPM) test germination. 10...

Immature avian sympathetic neurons are able to proliferate in culture for a limited number of divisions albeit expressing several neuron-specific properties. The effect of avian retroviral transfer of oncogenes on proliferation and differentiation of sympathetic neurons was investigated. Primary cultures of 6-d-old quail sympathetic ganglia, consisting of 90% neuronal cells, were infected by My...

background: it has been demonstrated that performing a mock embryo transfer (et) significantly improves the pregnancy rate in ivf cycles. the mock et could be performed prior to the stimulation cycle or immediately before real et. the problem of the first procedure is the possibility of variation in the uterine position and or cervico�uterine angle. objective: the purpose of this study was to d...

background: natural endometrium in frozen-thawed embryo transfer (fet) may have some benefits upon implantation in patients with repeated implantation failure (rif). it might be due to possible differences between natural and stimulated endometrial growth factors and cytokins secretions. objective: the objective of this study was to compare the pregnancy rate of fet on modified natural cycle ve...

in vitro maturation (ivm) is a method that immature oocytes in antral follicles are extracted and matured in laboratry conditions. this review has attempted to provide the current knowledge and recent findings in in vitro maturation of oocytes and highlights the most important factors involved in this process. the review is based on literature reports and the author’s experience. in ivm cycles,...