Most anesthetics have an immuno-suppressive effect on cellular and neurohumoral immunity, and research shows that total intravenous anesthesia (TIVA) with propofol has a greater immuno-protective effect than inhalational anesthesia in human medicine. However, in veterinary clinics, these effects remain ambiguous. To clarify the details, we focused on propofol and isoflurane, investigating clini...

Targeting drugs to their sites of action is still a major challenge in pharmaceutical research. In this study, polylactic-co-glycolic acid (PLGA) immuno-nanoparticles were prepared for targeting invasive epithelial breast tumour cells. Monoclonal antibody (mAb) was used as a homing ligand and was attached to the nanoparticle surface either covalently or non-covalently. The presence of mAb on th...

An antigen detection system, termed immuno-polymerase chain reaction (immuno-PCR), was developed in which a specific DNA molecule is used as the marker. A streptavidin-protein A chimera that possesses tight and specific binding affinity both for biotin and immunoglobulin G was used to attach a biotinylated DNA specifically to antigen-monoclonal antibody complexes that had been immobilized on mi...

BACKGROUND Definitive diagnosis of infestation with Angiostrongylus cantonensis is difficult because the parasitic nematode is undetectable in the cerebrospinal fluid (CSF) of one-half of afflicted patients and the diagnostic sensitivity of ELISA for circulating worm antigens in patient sera is low. We studied immuno-PCR as a diagnostic tool. METHODS We studied 30 controls and 60 afflicted pa...

Mesenchymal stromal cells (MSCs) possess both immuno-privileged and immuno-inhibitory properties that contribute to their therapeutic effects. Ex vivo expansion is required to obtain sufficient cells for therapy, but might also alter their immunological properties. To date there has been no systematic study of MSC immunobiology during extended culture. Here, we demonstrate that both immuno-priv...

Major surgery induces an immuno-inflammatory response accompanied by oxidative stress that may impair cellular function and delay recovery. The objective of the study was to investigate the effect of an enteral supplement, containing glutamine and antioxidants, on circulating levels of immuno-inflammatory markers after major gastrointestinal tract surgery. Patients (n 21) undergoing major gastr...

The monitoring of salivary cortisol as a key biomarker of an individual's stress response has been increasingly focused on. This paper describes the development of a novel cortisol immuno-assay method based on an indirect competitive method using a commercially available surface plasmon resonance instrument. The surface of an Au chip was modified with PEG6-COOH aromatic dialkanethiol self-assem...

A 60-year-old Japanese man had developed widespread erythemas with bullae on his trunk and extremities. He was diagnosed as having BP on the basis of the skin manifestations, histopathological ¢ndings and the results of immuno£uorescence staining. The serum was found to have an antibody titre of 1:60 on cryostat sections of guinea pig oesophagus substrate, and showed a typical basement membrane...

Biological processes occur at distinct but interlinked scales of organization. Yet, mathematical models are often focused on a single scale. Recently, there has been a significant interest in creating and using models that link the withinhost dynamics and population level dynamics of infectious diseases. These types of multi-scale models, called immuno-epidemiological models, fall in four categ...

In this paper a recursive immuno inspired algorithm is proposed to identify time variant discrete multivariable dynamic systems. The main contribution of this paper has as starting point the idea that a multivariable dynamic system state space model can be seen as a point in a space defined by all possible matrices quadruples that define a state space model. With this in mind, the time variant ...