identification of acid-fast bacilli (afb) in sputum or tissue samples is among definite diagnostic methods of tuberculosis. however, this method of diagnosis is restricted by certain limitations.   serologic diagnosis of tuberculosis (tb) has been used for a long time. the aim of this study was to determine the sensitivity and , specificity of antigen 60 (a60) igg, iga, igm test results in tb d...

Despite the paradigm that carbohydrates are T cell-independent antigens, isotype-switched glycan-specific immunoglobulin G (IgG) antibodies and polysaccharide-specific T cells are found in humans. We used a systems-level approach combined with glycan array technology to decipher the repertoire of carbohydrate-specific IgG antibodies in intravenous and subcutaneous immunoglobulin preparations. A...

In previous experimental animal studies it has been demonstrated that antibody depletion is not followed by increased antibody synthesis. To assess whether these results are conferrable to antibody-depleted humans, we measured free light chains (flcs) as markers of current antibody synthesis in 8 patients treated with immunoadsorption (IA) therapy. Specific and bulk immunoglobulin levels were o...

By immunoelectrophoresis at least three immunoglobulins, IgG, IgA and IgM can be recognized but quantitative determination of each immunoglobulin has been hampered by methodological difficulties. Recently, however, Fahey and McKelvey described in details an antibody-agar plate method for this purpose. While the author was following their method, it way found that less antiserum was required by ...

Case presentation: Ten year-old female presented whit visual loss and ocular pain with extraocular movements in the left eye papilledema. After 15 days, it progressed to right eye. No other neurological symptoms were observed. The case was investigated optical nerve magnetic resonance imaging (MRI), which evidenced enhancement of perineural involvement, brain spinal cord MRI without demyelinati...

Immunoglobulin (Ig) G, IgM, IgA and IgG subclass (IgG1, IgG2, IgG3, IgG4) concentrations were determined in more than 500 healthy Turkish children using nephelometric technique. These parameters were thought to be highly varied for different ethnic groups because of environmental and genetic factors. Methodology used in previous studies has been reported to affect age-related normal values. Ser...

Dried blood spot samples from mothers and their offspring attending the obstetric and pediatric departments of two hospitals in Lahore, Pakistan, were tested for antibodies to hepatitis C virus (HCV). The seroprevalence of HCV in the women was 6.7% (95% confidence interval [CI], 4.3 to 9.1), and that in the children was 1.3% (95% CI, 0.34 to 2.26). Four anti-HCV immunoglobulin G (IgG)-positive ...