Oocyte in vitro maturation (IVM) is an assisted reproductive technology in which oocytes are retrieved from the antral follicles of unstimulated or minimally stimulated ovaries. IVM of human oocytes has emerged as a promising procedure. This new technology has advantages over controlled ovarian stimulation such as reduction of costs, simplicity, and elimination of ovarian hyperstimulation syndr...

Address correspondence to: L.A. Miranda. Laboratorio de Ictiofisiología y Acuicultura. Instituto de Investigaciones Biotecnológicas Insituto Tecnológico de Chascomús (IIBINTECH), Chascomús (B7130IWA), Provincia de Buenos Aires, ARGENTINA. E-mail: [email protected] Received on April 1, 2005. Accepted on June 1, 2005. The pejerrey Odontesthes bonariensis is an atherinid fish native of the in...

The present study examined the kinetics of glutathione (GSH) concentration during maturation and after fertilization in pig oocytes and its relevance to the ability of pig oocytes to form a male pronucleus after in vitro fertilization. The GSH concentration was significantly higher in pig oocytes matured in Waymouth medium than in pig oocytes matured in either modified (m) TCM-199 or mTLP media...

The methylation status of sperm DNA differs between individual bulls. However, the relationship between methylation status and bull sperm parameters is not well elucidated. The present study investigated genome-wide methylation profiles at 450,000 CpG sites in bull spermatozoa by using a human DNA methylation microarray. Semen samples from three adult Japanese Black bulls with different in vitr...

There are two groups of MADS intervening keratin-like and C-terminal (MIKC)-type MADS box genes, MIKC(C) type and MIKC* type. In seed plants, the MIKC(C) type shows considerable diversity, but the MIKC* type has only two subgroups, P- and S-clade, which show conserved expression in the gametophyte. To examine the functional conservation of MIKC*-type genes, we characterized all three rice (Oryz...

Background: Parthenogenesis is the production of an embryo from a female gamete in the absence of any contribution from a male gamete, with or without the eventual development into an adult. In vivo, mammalian parthenogenesis is a rare event. Parthenogenesis can be efficiently induced in vitro with a variety of mechanical, chemical, and electrical stimuli in several species. Hydrostatic pressur...

the aim of the present study was to find the effects of pre and post vitrification taxol treatment on bovineimmature cumulus oocyte complexes (cocs). bovine ovaries were collected from the local slaughterhousein a container with 30-37°c physiologic saline and supplemented with antibiotics. the grade a aspiratedcocs were assigned to 6 experimental groups; the 1st group was control (n=100) in whi...

in traditional medicine, mentha spicata is widely used for many diseases, especially in  digestive system. it has anti-androgenic effects thus can be used by women experiencing polycystic ovary syndrome (pcos). also, it has shown to possess antioxidant properties. although beneficial, studies have shown its detrimental effect on some tissues. the present study was carried out to determine the e...