I . Details are given for the construction and use of an apparatus for the incubation of rumen liquor in semi-permeable sacs inside the bovine rumen. It was found practicable, using one animal, to incubate simultaneously samples of strained diluted rumen liquor in three identical vessels, each of 100--120 ml capacity, for periods of up to 23 h. 2. The optimal conditions for experiments of this ...

A method is described that obviates the need for vacuum and tanks of gas mixtures for anaerobiosis in the anaerobic incubator.

ABSTRACT: The objectives of this study were to assess the effects i) pre-incubation storage time Pekin duck eggs on incubation parameters and ii) different levels in ovo injection with maltodextrin duckling weight. was divided into two experiments using hatching Cherry Valley SM2 hens egg-laying ages between 31 40 weeks. In experiment I, 8,820 subjected periods (one-seven days). For II, 120 wei...

A stable xylan suspension was prepared and characterized. Hydrolysis of the particles converts them into soluble fragments, thereby lowering the turbidity of the suspension. The small volume of the assay mixture, the short incubation time required, and the simplicity of the procedure permit the rapid analysis of many samples. Furthermore, the procedure can be used to assay xylanase activities i...

A plaque reduction method for measuring lymphocytic choriomeningitis virus-sensitizing antibody in human serum is described. One volume of virus and one volume of serially diluted human serum were mixed and incubated for 2 h at 37 degrees C. One volume of suitably diluted anti-human immune globulin antiserum was added and incubation continued for 0-5 h. Residual infectivity was then determined ...

Insulin concentrated from acid/ethanol extracts of IMg lymphocytes by the use of octadecylsilyl (ODS) silica cartridges averaged 0.13 ng/ml cell volume compared with the 0.12 ng insulin/ml cell culture medium. The insulin content of these cells appears to be less than 1% of previously reported values and probably could be accounted for by concentration of insulin from the incubation medium on c...

After incubation in isotonic KCl, dog red blood cells can be separated by centrifugation into subgroups which assume different cell volumes and possess different transport characteristics. Those red cells which swell in isotonic KCl exhibit a higher permeability to K and possess a greater volume dependence for transport of K than those red cells which shrink. A high Na permeability characterize...

Background. Microcinephotography was used to study a model of persistent truncus arteriosus created in chick embryos by ablation of premigratory neural crest destined for the third and fourth aortic arch arteries as well as the septum of the cardiac outflow tract. Methods and Results. Twenty-five control embryos and 105 of 202 experimental embryos were filmed on day 3 of incubation and then rei...