Methylene blue is the most extensively used chemical indicator of anaerobiosis. Its use as such an indicator was reported early in the literature (Smith, Wilder Quarter Century Book, p. 187, 1893). Techniques such as hanging strips of methylene blue-impregnated gauze inside an anaerobic jar were described by van Reimsdijk (Ned. Tijdschr. Geneesk. 66:1423, 1922). Present-day microbiologists ofte...