Nucleotide sequences of the VP2 hypervariable region (VP2-HVR) of 10 infectious bursal disease viruses detected in indigenous and exotic chickens in Zambia from 2004 to 2005 were determined. Phylogenetic analysis showed that the viruses diverged into two genotypes and belonged to the African very virulent types (VV1 and VV2). In the phylogenetic tree, strains in one genotype clustered in a dist...

Antigen Capture-ELISA based on eight different neutralizing mouse monoclonal antibodies (Mabs) was used to study Polish infectious bursal disease viruses (IBDVs) isolated from two epidemics on the turn of 70/80s (early IBDV) and in the 90s (recent IBDV). They were compared to the Faragher 52/70 (F52/70) reference strain of European classical serotype 1 IBDV and to the 89/163 (typical) and 91/16...

A reverse genetics system for birnavirus, based on synthetic transcripts of the infectious bursal disease virus (IBDV) genome, was recently developed (E. Mundt and V. N. Vakharia, Proc. Natl. Acad. Sci. USA 93:11131-11136, 1996). To study the function of the 17-kDa nonstructural (NS) protein in viral growth and pathogenesis, we constructed a cDNA clone of IBDV segment A in which the first and o...