Introduction of ?-N-methylated non-proteinogenic amino acids into peptides can improve their biological activities, membrane permeability and proteolytic stability. This is commonly achieved, in nature the lab, by assembling pre-methylated acids. The more appealing route methylating amide bonds challenging. Biology has evolved an ?-N-automethylating enzyme, OphMA, which acts on fused to its C-t...

background: enterocin-p of enterococcus faecium p13 (entp) is of great interest as a food preservative and medicine due to its non-toxicity and broad antimicrobial spectrum in various ph, as well as its excellent thermal stability. however, recombinant production of entp is still in laboratory because of low productivity and complex purification process. objectives: in this study, we aimed to d...