A kinetic metabolic model describing Catharanthus roseus hairy root growth and nutrition was developed. The metabolic network includes glycolysis, pentose-phosphate pathway, TCA cycle and the catabolic reactions leading to cell building blocks such as amino acids, organic acids, organic phosphates, lipids and structural hexoses. The central primary metabolic network was taken at pseudo-steady s...

OBJECTIVE Many cell types in atherosclerotic lesions are thought to have various biological abnormalities, such as impaired lipid homeostasis and slow cell proliferation, which may be related to senescence at cellular and individual levels. One of the common characteristics of senescent cells in vitro is the alteration of actin cytoskeletons, which have been reported to be involved in the intra...

background: hypertensive patients are highly susceptible to oxidizability. although hypertension is common among iranians, few data are available about lipid oxidation status in the involved people. the aim of this study was to evaluate the relationship between serum lipids levels and susceptibility of lipids to oxidation in hypertensive patients. materials and method: fasting serum lipids and ...

In endometriosis, an aberrant immune response in the peritoneal environment is evident, but underlying mechanism remains unclear. recent years, some emerging data support important role for changes intracellular metabolic pathways of cells controlling their function. this study, we aim to investigate phenotypic and immunometabolism profiling fluid endometriosis patients. By flow cytometry, our ...

Methylmercury (MeHg) is a ubiquitous contaminant largely found in aquatic environments, especially species at high trophic level such as salmonids. The aim of this study was to evaluate the effects MeHg on adipocyte differentiation and lipid metabolism rainbow trout. Primary cultured preadipocytes were exposed increasing concentrations during six days with or without hormonal cocktail. Main res...

SCOPE First- and second-generation antipsychotics (FGAs and SGAs, respectively), both inhibit cholesterol biosynthesis and impair the intracellular cholesterol trafficking, leading to lipid accumulation in the late endosome/lysosome compartment. In this study we examined if curcumin, a plant polyphenol that stimulates exosome release, can alleviate antipsychotic-induced intracellular lipid accu...

The large family of mammalian TRP (transient receptor potential) ion channels encompasses diverse sensory functions. TRP proteins consist of six transmembrane domains, with a pore-loop motif between the fifth and sixth domains and cytosolic N- and C-termini. The intracellular strands not only interact with various proteins and lipids, but also include essential multimerization regions. This rev...

Many signaling pathways converge on and regulate phosphoinositide 3-kinase (PI3K) enzymes whose inositol lipid products are key mediators of intracellular signaling. Different PI3K isoforms generate specific lipids that bind to FYVE and pleckstrin homology (PH) domains in a variety of proteins, affecting their localization, conformation, and activities. Here we review the activation mechanisms ...

With the multiparametric measurement of individual cells at a rate of several thousand per second, flow cytometry offers a rapid method for the detailed analysis of microorganisms. This technique provides quantitative data for cell size or granularity through light scattering signals and via fluorescence signals quantitative information on the expression of surface antigens, or intracellular co...