Immunohistochemical distributions of cathepsins D and E were determined in normal mucosa, metaplastic, dysplastic, and cancerous lesions of the human stomach. Cathepsins D and E were localised in the foveolar epithelium and parietal cells of the normal gastric mucosa, but their intracytoplasmic distributions were different - cathepsin E distribution was even and diffuse in the cytoplasm while c...

The ICSI has become method of choice to achieve fertilization. Fertilization is possible in cases in which the sperm motility and ability to penetrate the zona pellucida are impaired. Injection is possible with sperm obtained from ejaculation, microsurgical epididymal sperm aspiration (MESA), percutaneous epididymal sperm aspiration (PESA), or testicular sperm extraction (TESE). In addition, in...

The fine structure of the intracytoplasmic organelles of mycobacteria was studied by means of electron microscopy of ultrathin sections. A well-preserved nuclear apparatus was obtained by fixation with OsO(4) in acetate-veronal buffer, containing calcium and tryptone, or in collidine-HCl buffer, followed by uranyl-acetate treatment and embedding in araldite. A low density nuclear region was fil...

Intracytoplasmic spermatozoid injection (ICSI) is one of the commonest methods used in assisted reproductive technologies human medicine. However, this procedure has low efficacy for bovines, mainly because insufficient activation oocytes after microinjection. One most effective activating considered to be use phosphorus calcium, though optimal concentration activator and its effect on pre-impl...

Selection of spermatozoa is one the most important factors that influence embryonic development and clinical outcome in intracytoplasmic sperm injection (ICSI). Intracytoplasmic morphologically selected (IMSI) a technique selects only according to their features under high magnification. However, there another selection uses hyaluronic acid (HA) select physiologically mature (HA-ICSI). The purp...