In the present study we examined the effect of histamine injection on neuropeptide release in vivo in rats. Rats were injected with histamine either into the left knee joint or intraperitoneally. Concentrations of substance P (SP)-, neurokinin A (NKA)-, calcitonin gene-related peptide (CGRP)- and neuropeptide Y (NPY)-like immunoreactivity (-LI) were examined in the knee joint perfusates and cer...

L forms induced from two strains of group A Streptococcus were inoculated intraperitoneally and intravenously into conventional and germ-free mice. After intravenous injection, streptococcal L forms disappeared very quickly from the blood, whereas, after intraperitoneal injection, it was possible to isolate them as long as 25 days after inoculation. Observations suggest that a certain degree of...

After an intraperitoneal injection of 100 mumol of methionine to rats, there is rapid oxidation of the methyl group of hepatic 5-methyltetrahydrofolate to formate and CO2. Recovery of the methylfolate level starts 2.5 h after the methionine injection, when the hepatic methionine level and the S-adenosylmethionine/S-adenosylhomocysteine ratio have returned to baseline values. S-Adenosylmethionin...

C57BL/6J mice rendered hypersensitive to Blastomyces dermatitidis were protected from the lethal effects of a blastomyces infection. This protection was observed following a lethal intraperitoneal challenge with viable cells 15 days after subcutaneous inoculation with 3.9 x 10(4) viable cells. Delayed hypersensitivity was induced in C57 mice by two injections of Merthiolate-killed cells in adju...

Phage therapy has been suggested as a potential treatment for highly antibiotic-resistant bacteria, such as the species of the Burkholderia cepacia complex (BCC). To address this hypothesis, experimental B. cenocepacia respiratory infections were established in mice using a nebulizer and a nose-only inhalation device. Following infection, the mice were treated with one of five B. cenocepacia-sp...

INTRODUCTION The aim of this study was to investigate angiotensin II type 1 (AT1) receptors in rat bladder smooth muscle cells and alterations of AT1 receptors by diabetes mellitus and diuretic states. MATERIALS AND METHODS Diabetes and diuresis were induced in adult female rats by a single intraperitoneal injection of streptozotocin and feeding 5% sucrose in water. Cystometry was performed o...

In order to investigate whether earthworm fibrinolytic enzyme III-1 (EFE-III-1) isolated from Lumbricus rubellus is capable of transporting into blood through intestinal epithelium and keeping its biological function in circulation, we have raised an antibody against EFE-III-1. The immunological results showed that 10-15% of intact EFE-III-1 was absorbed by the intestinal epithelium with the in...

The toxic and carcinogenic actions of four methylnitrosamine or -amide compounds, given as a single intraperitoneal injection to newborn and adult inbred Swiss mice, were examined. Toxicity was approximately directly proportional to the stability of the three compounds capable of methylating macromolecules in vitro. Methylnitrosourea induced a high incidence of thymic lymphomas in adults and ne...

Introduction Effective and safe anesthesia for rodents has long been a leading concern among biomedical researchers. Intraperitoneal injection constitutes an alternative to inhalant anesthesia. Purpose The aim of this study was identify safe, reliable, effective postoperative analgesia protocol laboratory rats exposed painful procedures. Material methods Twenty-seven female Wistar in ongoing th...

The present work focused on role of the immune organ in the thymus of Mystus gulio. Haemopoietic tissue was found mainly in the spleen, head kidney, thymus and a small amount occurred in the mesonephros. The clearance of the intraperitoneally injected colloidal carbon was carried out. The phagocyte uptake of carbon, after its intraperitoneal injection. It was first detected in the thymus at 30 ...