Serum from patients who have suffered acute pancreatitis contains P3, an isoenzyme of pancreatic-derived amylase (EC 3.2.1.1). Heretofore, complete resolution of P3 from the major salivary isoenZyme in serum, Si, has not been possible, thus compromising the diagnostic potential of P3 for pancreatitis. I describe an electrophoretic method for the essentially complete resolution of P3 from S1 by ...

Starch gel electrophoresis of homogenates from human stomach mucosa resolves three alcohol dehydrogenase (ADH) forms: the anodic chi-ADH (class III), the cathodic gamma-ADH (class I), and a new form of slow cathodic mobility that has not been previously characterized. In this work, we describe the purification in three chromatographic steps and the physical and kinetic characterization of this ...

Diabetes mellitus is a group of metabolic disorders that share with symptoms hyperglycemia led to elevated free radical activity. Hyperglycemia linked higher level (ADA), which one the factors cause oxidative stress by creating reactive oxygen species (ROS), leads insulin resistance. This study aimed determine relationship between serum total adenosine deaminase(TADA) and its isoenzyme (ADA1and...

This semi-automated colorimetric assay for the MB isoenzyme of creatine kinase (EC 2.7.3.2) is based on a monoclonal antibody ("Conan-MB") specific for this isoenzyme and is a modification of a previously published method (Vaidya et al., Clin Chem 1986;32:657-63). A 0.64-cm bead coated with 2 to 3 micrograms of antibody is incubated with 100 microL of serum and 10 microL of 0.2 mol/L beta-merca...

The view is advanced that it is important to fix the stage of development corresponding to the pattern of expression of oncodevelopmental proteins in neoplastic transformation and neoplasia. From a map of development focused on events beginning with the zygote and ending with the establishment of fetus and placenta, it is possible to explain why certain develop mental gene products are restrict...

The study was aimed at assessing the total enzyme activity and the profile of breast cancer and dysplasia on the human material. In addition, the validity of data was evaluated from the aspect of improving diagnostics. Lactate dehydrogenase activity, as well as the profile of its isoenzymes, pyruvate kinase and hexokinase, were measured. The study included 60 samples of breast cancer, out of wh...

HeLa TCRC-1, is a monophenotypic cell line for the production of the carcinoplacental Regan isoenzyme of alkaline phosphatase. It has been previously reported that, when this cell line is grown in immunosuppressed rats, there is an alteration of phenotypic expression in that an isoenzyme that is referred to as oncoamnion (FL) becomes the dominant enzyme form. This report describes the isoenzyme...

system of Jervis [7] is used here.) Previous electrophoretic examination of the LDH of most other plants was carried out with extracts from leaves [1,2j. Such a study of potato leaves would not have revealed the existence of isoenzymic forms in this species. We have, therefore, examined extracts from various regions of a number of plants: roots, stem-base, upper stems, leaves and any special ti...

the effects of different storage temperatures and times on germination rate, malondialdehyde (mda), superoxide dismutase (sod), peroxidase (pod) and catalase (cat) activities in jatropha curcas l. seed were investigated. the results showed that germination rates of seeds stored at room temperature and 4°c for 1 month was significantly higher than those stored for 3 months at -20 and -80°c. mda ...

background: cutaneous leishmaniasis (cl) with diverse clinical manifestations is prevalent and remains a major public health problem in iran and its incidence has been doubled over the last decade. the present study is about the potential role of rodents in the epidemiology of cl in kharameh district in shiraz, southern iran. methods: from april 2004 to april 2005, a total of sixteen rodents we...