We examined the effect of storing human plasma or extracts of prostate at -90 degrees C on the activity of creatine kinase and lactate dehydrogenase and isoenzyme distribution. Enzyme activities were unaltered during storage for as long as six weeks. If these preparations were thawed only once at 2 to 4 degrees C, they could be stored for as long as 165 days at -90 degrees C with no change in i...

Porcine lactic dehydrogenase (L.D.H.) has been found in the serum of four patients in hepatic coma treated by extracorporeal porcine liver perfusion. A comparison between the porcine L.D.H. isoenzyme pattern in the patients' serum and that in porcine serum and liver extract indicates that the porcine L.D.H. is derived from the pig liver. This finding reflects damage to the pig liver cells susta...

An enzymatic, immunochemical test system has been developed which allows quantitation of minute amounts of the Regan isoenzyme of alkaline phosphatase. The basis of the method is the concentration (tenfold) of the Regan isoenzyme by reaction with a monospecific antiserum to placenta! alkaline phosphatase, insolubilized by polymerization with ethyl chloroformate. This concentration is achieved b...

Isoenzyme and random amplified polymorphic DNA (RAPD) markers were used to characterize the genetics of geographic variation among population samples of Ascaris suum from midwestern localities. Independent estimates of fixation indices (FST) based on isoenzyme and RAPD markers showed the same general patterns of differentiation and substantial statistical correlation (r = 0.70). Of the total es...

The structural relationships among human alkaline phosphatase isoenzymes from placenta, bone, kidney, liver and intestine were investigated by using three criteria. 1. Immunochemical characterization by using monospecific antisera prepared against either the placental isoenzyme or the liver isoenzyme distinguishes two antigenic groups: bone, kidney and liver isoenzymes cross-react with anti-(li...

CLINICAL CHEMISTRY, Vol. 30, No.8, 1984 1425 bined isoenzyme analysis using the DuPont aca analyzer to measure CKMB and LDH1. InBiologieProspective. ComptRenddu5 Colloq de Pont-d-Mou8son, MM Galteau, G Sleet, J Henny, Eds., Masson, Paris, 1983, pp 627-630. 8. McCoy MT. Aguanno JJ, Ritzms nn SE. Lactate dehydrogenase isoenzyme 1. A rapid assay for ischemic myocardial disease. Arch Intern Med 143...

Medical records of 53 burn and trauma patients were reviewed to assess the possibility of myocardial damage. Except for electrophoretically detectable creatine kinase MB isoenzyme, none showed evidence of myocardial injury. Lactate dehydrogenase isoenzyme tests, electrocardiograms, myocardial pyrophosphate scans, clinical course, and results of (two) autopsies were all negative for myocardial n...

The brain isoenzyme of creatine kinase (CK) (EC 2.7.3.2) has been identified in the serum of 21 of 950 patients tested. Samples were received over a period of about 20 months. Analysis was performed by electrophoresis on agarose gel with isoenzyme bands visualized by fluorescence after enzyme coupled reactions producing NADH. Specialized substrate preparations were used to rule out the possibil...

HeLa Cells have been found to produce a placental form of alkaline phosphatase (1, 8, 17) which is very similar to the Regan isoenzyme, a carcinoplacental antigen found in cancer patients (7-9) . In order to investigate the nature of gene expression of carcinoplacental proteins, HeLa cells were cloned, and a subline (HeLa TCRC-1), monophenotypic for the Regan isoenzyme was selected . The proper...