The genus Harpagophytum has two species: H. procumbens which is an important medicinal plant in southern Africa, and H. zeyheri. Genetic diversity in 96 samples, obtained by germinating seeds collected from Botswana, was assessed using six inter-simple sequence repeat (ISSR) and 10 random amplified polymorphic DNA (RAPD) primers. These DNA markers yielded a total of 138 polymorphic bands. Polym...

Sequence-related amplified polymorphism (SRAP) and inter-simple sequence repeat (ISSR) markers were used to assess the genetic diversity within and among 15 natural populations of Stipa bungeana from the Loess Plateau of China. Using 15 SRAP primers, 504 (99.80%) polymorphic loci were detected, and 372 polymorphic loci (96.12%) were identified using 15 ISSR primers. At the species level, the S....

of high-quality vegetable and industrial oil. 9 RAPD primers, 6 AFLP primer combinations and 12 agro-morphological traits were used to assess the genetic diversity of 20 accessions of saf flower representing global germplasm variability. Jacquards’ similarity coef ficient were used to understanding the genetic relationships among ccessions, for AFLP and RAPD markers and Euclidian similarity coe...

This article refers to viewing the role of molecular markers during analyzing genome plants and their importance in plant biotechnology. In recent years, we observed techniques programs for improving breeding preserving genetic resources has been observed, biochemical indicators which represent basic material through determining diversity between genotypes it is never affected by external surro...

Urtica dioica is an important medicinal plant which is widely distributed in Mazandaran province (North of Iran). In this study for the first time Amplified Fragment Length Polymorphism (AFLP) and Inter-simple Sequence Repeat (ISSR) markers were used for detection of genetic polymorphism in Mazandaran nettle. Ten AFLP primer combinations and seventeen ISSR markers were utilized. AFLP produced 830...

In this study, we report a simple procedure for developing and using new types of polymerase chain reaction (PCR) primers, named ‘‘high-frequency oligonucleotides–targeting active genes’’ (HFO-TAG). The HFOTAG primers were constructed by first using a ‘‘practical extraction and report language’’ script to identify oligonucleotides (8, 9, and 10 bases) that exist in high frequency in 4700 expres...