The biodiversity assessment of different taxa of the genus Caulerpa is of interest from the context of morphological plasticity, invasive potential of some species and biotechnological and pharmacological applications. The present study investigated the identification and molecular phylogeny of different species of Caulerpa occurring along the Indian coast inferred from tufA, rbcL, 18S rDNA and...

Smc5/6, a member of the conserved SMC family of complexes, is essential for growth in most organisms. Its exact functions in a mitotic cell cycle are controversial, as chronic Smc5/6 loss-of-function alleles produce varying phenotypes. To circumvent this issue, we acutely depleted Smc5/6 in budding yeast and determined the first cell cycle consequences of Smc5/6 removal. We found a striking pri...

Following chromosome duplication in S phase of the cell cycle, the sister chromatids are linked by cohesin. At the onset of anaphase, separase cleaves cohesin and thereby initiates sister chromatid separation. Separase activation results from the destruction of its inhibitor, securin, which is triggered by a ubiquitin ligase called the anaphase-promoting complex (APC). Here, we show in budding ...

In the yeast Saccharomyces cerevisiae, one determinant of aging or life span is the accumulation of extrachromosomal copies of rDNA circles in old mother cells [1]. The production of rDNA circles depends upon intrachromosomal recombination within the rDNA tandem array, a process regulated by the protein Sir2 (Sir2p). Together with Sir1p, Sir3p, Sir4p and Orc1p, Sir2p is also involved in transcr...

The Saccharomyces cerevisiae ribosomal DNA (rDNA) locus is known to exhibit greater instability relative to the rest of the genome. However, wild-type cells preferentially maintain a stable number of rDNA copies, suggesting underlying genetic control of the size of this locus. We performed a screen of a subset of the Yeast Knock-Out (YKO) single gene deletion collection to identify genetic regu...

C A T G C A A G T C G A A C G G A A A G G C C T C T T C G G A G G T A C T C G A G T G G C G A A C G G G T G A G T A A C M. avium sqv. I . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M. avium ssp. avium sqv. II . . . . . . . . . . . . . . . . . . . . . . . C . . . . . . G . . . . . . . . . . . . . . . . . . . . . . . . . ....

The nucleotide sequence of 23S rDNA from Zea mays chloroplasts has been determined. Alignment with 23S rDNA from E.coli reveals 71 percent homology when maize 4.5S rDNA is included as an equivalent of the 3' end of E.coli 23S rDNA. Among the conserved sequences are sites for base modification. Chloramphenicol sensitivity and ribosomal subunit interaction. A proposal for the base pairs formed be...

Aim Fungi make up a large, ecologically important group of eukaryotes that has been neglected in historical biogeography. In this study, molecular phylogenies and molecular clock dating were used to infer historical biogeography of the shiitake genus, Lentinula Earle, which occurs in both the Old World and New World. The major goals of this study were to determine if the Old World and New World...

background: strongyloides stercoralis , the etiological agent of strongyloidiasis, is one of the most common parasitic nema­tode with the unique ability to complete its life cycle and proliferate within the host. although it is an endemic parasite in iran, no molecular characterization is available on isolates originated from the country. therefore, this study was con­ducted for molecular ident...