OBJECTIVE We aim to evaluate the effectiveness of the brush biopsy technique using OralCDx ® (OralScan Laboratories Inc., Suffern, NY) as a new method for early diagnosis and control of a "potentially malignant disorder" such as oral leukoplakia. DESIGN OF THE STUDY We performed a study in which samples were taken using OralCDx ® on 24 patients who visited the Master of Oral Medicine, Oral Su...

All-trans-retinoic acid (RA) regulates epithelial differentiation and growth through activation of specific nuclear RA receptors (RARs). Because high-rate metabolism largely impairs the biological efficacy of RA, we have sought for compounds capable of inhibiting the metabolic breakdown of the retinoid. This study identifies R115866 as a novel inhibitor of the cytochrome P450 (CYP)-mediated met...

Harlequin ichthyosis (HI), which is the most severe genodermatosis, is caused by loss-of-function mutations in ABCA12, a member of the ATP-binding cassette transporter family. To investigate the pathomechanism of HI and the function of the ABCA12 protein, we generated ABCA12-deficient mice (Abca12(-/-)) by targeting Abca12. Abca12(-/-) mice closely reproduce the human HI phenotype, showing mark...

PURPOSE Meibomian gland dysfunction (MGD) may be the leading cause of dry eye syndrome throughout the world. However, the precise mechanism(s) underlying the pathogenesis of this disease is unclear. This study was conducted to identify meibomian gland genes that may promote the development and/or progression of human MGD. METHODS Lid tissues were obtained from male and female MGD patients and...

BACKGROUND The clinical relevance of the carbohydrate antigen Sialyl Lewis a (SLea) as a serum tumor marker in diagnosis and follow-up treatment is unquestioned in a broad spectra of human carcinomas. Overexpression of this antigen is combined with poor prognosis and malignant relapse. The aim of our study was the systematic investigation of SLea expression in squamous cell carcinoma of the lar...

PURPOSE The aim of this study was to evaluate a human meibomian gland epithelial cell line (HMGEC) as a model for meibomian gland (patho)physiology in vitro. METHODS HMGEC were cultured in the absence or presence of serum. Sudan III lipid staining, ultrastructural analysis and lipidomic analyses were performed. Impedance sensing, desmoplakin 1/2 mRNA and cytokeratin (CK) 1, 5, 6, 14 levels we...

An 81-year-old female consulted us because of an ulcerated tumor in her left breast of several years’ duration. Partial biopsy showed that the tumor consisted of anaplastic squamous cells, indicating squamous cell carcinoma from unknown origin. CT images demonstrated tumors in the left breast, liver and bile duct. However, further investigation was not possible due to the patient’s refusal. To ...

This study describes changes in the pattern of microridges, keratinization, desquamation, secretion, leukocyte infiltration as well as the increasing number of mitotic cells in the vaginal epithelium of Calomys callosus during the oestrous cycle. In proestrus, the epithelium is squamous and stratified with a fine layer of keratin and it is overlain by secretory prismatic cells. In oestrous, the...

OBJECTIVES We assessed the relationship between DNA ploidy and histopathologic parameters and its effect on prognosis in patients with squamous cell carcinoma of the larynx. PATIENTS AND METHODS Surgical sections of 44 patients who were treated for squamous cell carcinoma of the larynx were studied. Image cytometry measurements were performed on nuclear suspensions derived from paraffin-embed...