The study was focused on the effect of drought and waterlogging stresses in two-year pot experiments peat substrate content glucose, fructose sucrose free amino acids potato tubers four cultivars (yellow-fleshed Laura, Marabel, Milva blue-fleshed Valfi) after 71 days exposure to conditions (BBCH 909). Drought increased levels fructose, three except for cv. Laura. stress l-proline (+248.4%), l-h...

Inbred weanling rats were maintained on diets containing low (6%), medium (lS%), and high (40%) levels of proteins. After 45 days they were killed and the activities of histidine ammonia lyase, urocanase, and histidine pyruvate aminotransferase in liver were assayed. Histidine ammonia lyase increased with age of the rat, and the increase was proportional to the level of protein in the diet. Ani...

The argininosuccinate lyase activity of duck delta-crystallin was inactivated by diethyl pyrocarbonate at 0 degrees C and pH 7.5. The inactivation followed pseudo-first-order kinetics after appropriate correction for the decomposition of the reagent during the modification period. The plot of the observed pseudo-first-order rate constant versus diethyl pyrocarbonate concentration in the range o...

Heparinase production by Flavobacterium heparinum in complex protein digest medium, with heparin employed as the inducer, has been studied and improved. The maximum productivity of heparinase has been increased 156-fold over that achieved by previously published methods to 375 U/liter per h in the complex medium. Rapid deactivation of heparinase activity, both specific and total, was observed a...

Endosomal entrapment is one of the main barriers that must be overcome for efficient gene expression along with cell internalization, DNA release, and nuclear import. Introducing pH-sensitive ionizable groups into the polycationic polymers to increase gene transfer efficiency has proven to be a useful method; however, a comparative study of introducing equal numbers of ionizable groups in both ...

This 1H-NMR study provides experimental evidence for an intermolecular interaction between the dipeptide carnosine (beta-alanyl-L-histidine) and the purine nucleoside 5'-monophosphates 5'-AMP, 5'-GMP. From the observed upfield shifts of the purine nucleotide and imidazole proton resonances it is concluded that the interaction is of the stacking type and that it involves the purine base of the n...

A search for genes expressed more highly in mouse cumulus cells than mural granulosa cells by subtraction hybridization yielded Slc38a3. SLC38A3 is a sodium-coupled neutral amino acid transporter having substrate preference for l-glutamate, l-histidine, and l-alanine. Detectable levels of Slc38a3 mRNA were found by in situ hybridization in granulosa cells of large preantral follicles, but level...

objective(s) phosphatidate phosphohydrolase (pap) catalyzes the dephosphorylation of phosphatidic acid to yield pi and  diacylglycerol. two different forms of pap in rat hepatocyte have been reported. pap1 is located in cytosolic and microsomal fractions and participates in the synthesis of triacylglycerols, phosphatidylcholine, and phosphatidylethanolamine, whereas the other form of phosphatid...

et al., 1952). In liver disease or folic acid deficiency, abnormal quantities ofthe metabolites, urocanic acid and formiminoglutamic acid (Figlu), appear in the urine following an oral dose of the amino acid L-histidine (Luhby, Cooperman, and Teller, 1959; Spray and Witts, 1959; Kohn, Mollin, and Rosenbach, 1961; Carter, Schaffner, and Heller, 1960; Bennett and Chanarin, 1962; Merritt et al., 1...