fifty six holstein lactating cows (71 days in milk; 35 kg/day milk production) were employed in a complete block randomized design of repeated measurements. cows were randomly allocated to two treatments based on their parity. cows in group 1 were fed a balanced total mixed ration (600 g concentrate /kg dm and 400 g forages /kg dm) with 50 g rumen protected methionine (rpm) per head per day (me...

Efficient reproduction is important for optimal profitability on dairy operations. Unfortunately, most dairy farms do not attain optimal reproduction due to many factors related to management, health, and physiology of high-producing dairy cows. The physiology issues involved in reproduction of lactating dairy cows are complex but increasingly, the interactions between nutrition, the hormonal s...

Controlling heat stress (HS) is a global challenge for the dairy industry. In this work, an integrated metabolomics and lipidomics approach using (1)H nuclear magnetic resonance (NMR) and ultra-fast LC-MS in combination with multivariate analyses was employed to investigate the discrimination of plasma metabolic profiles between HS-free and HS lactating dairy cows. Here we provide the informati...

This review addresses the suggestion that the decline in dairy reproductive performance, as increasingly observed these days, may be due to a hampered process of metabolic adaptation in early lactating cows. In our opinion, adaptation to the negative energy balance is a gradual process. Because almost all cows do adapt in the long run, it is not possible to classify animals as adapted or non-ad...

the objective of the present study was to compare the efficacy of conventional and extendedintramammary (imm) therapy of persistent subclinical mastitis in lactating dairy cattle using nafcillinpenicillin-dihydrostreptomycin combination (npd). sixty-five dairy cows with 126 infected quarters were enrolled in the study. infected cows were allocated randomly to 1 of 3 different treatment regimens...

Heat stress (HS) damages the global dairy industry by reducing milk yields and quality, harming health, and damaging the reproduction of dairy cows, causing huge economic losses each year. However, an understanding of the physiological mechanism of HS lactating dairy cows remains elusive. Here, a metabolomics study using LC-MS and (1)H NMR spectroscopy was performed to analyze the metabolomic d...

The aim of this experiment was to investigate the effects of partial replacement of corn silage with long alfalfa hay and/or coarse chopped wheat straw on biochemical parameters in late lactating Holstein primiparous dairy cows. Twelve late lactating Holstein primiparous cows including four cows equipped with a rumen cannula, averaging 210 ± 20 days in milk and weighing 575 ± 50 kg were randoml...

________________________________________________________________________________ Abstract The objective of this study was to investigate the effects of inoculating Lactisil Maize, a dual-purpose inoculant, on the fermentation characteristics and nutritive value of high-moisture maize silage and the performance of lactating cows receiving the silage in their diets. Whole-crop maize was harvested...

Staphylococcus aureus is among the main etiologic agents of bovine mastitis. A total of 83 isolates of S. aureus from mammary glands of primiparous heifers were collected in the prepartum, calving and during lactation. For lactating cows, a total of 27 isolates of S. aureus from mammary glands were collected during lactation. The samples were taken in two dairy farms located in Sao Paulo State...

Physical and chemical characteristics of dietary ingredients and their interactions can have a large effect on dry matter intake (DMI) of lactating cows. Physical limitations caused by distension of the reticulo-rumen or other compartments of the gastrointestinal tract often limit DMI of high producing cows or cows fed high forage diets. Fermentation acids also limit DMI from a combination of i...