DNA ladder is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure to prepare a DNA ladder that consists of 10 fragments from 100 to 1000 bp. This protocol is a combination of routinely employed methods: cloning, PCR, and partial digestion with restriction enzymes. DNA fragments of 100 bp with...

An algorithm for modelling and simulating a virtual Jacob’s ladder is presented. Its motion is computed using our physically based simulator Iota. The model used to represent the Jacob’s ladder is dynamically restructured during the simulation via scripted events, but can also be performed interactively by the user. Modelling a Jacob’s ladder is achieved by reducing the problem to two dimension...

An effective intra-and inter-ladder charge-spin hamiltonian for the quarter-filled ladder compound α ′-NaV 2 O 5 has been derived by using the standard canonical transformation method. In the derivation, it is clear that a finite inter-site Coulomb repulsion is needed to get a meaningful result otherwise the perturbation becomes ill-defined. Various limiting cases depending on the values of the...

The Moore bound constitutes both an upper bound on the order of a graph of maximum degree d and diameter D = k and a lower bound on the order of a graph of minimum degree d and odd girth g = 2k + 1. Graphs missing or exceeding the Moore bound by ǫ are called graphs with defect or excess ǫ, respectively. While Moore graphs (graphs with ǫ = 0) and graphs with defect or excess 1 have been characte...

Ladder logic is a graphical language widely used to program Programmable Logic Controllers (PLCs). PLCs are found at the heart of most industrial control systems used in automation because they are robust, they are relatively easy to program and because they are a proven technology. However there is currently no means to measure the intrinsic properties and qualities of the code produced. This ...

Ladder networks are a notable new concept in the field of semi-supervised learning by showing state-of-the-art results in image recognition tasks while being compatible with many existing neural architectures. We present the recurrent ladder network, a novel modification of the ladder network, for semi-supervised learning of recurrent neural networks which we evaluate with a phoneme recognition...

William Blake’s iconic painting Jacob’s Ladder was inspired by the Old Testament story in the book of Exodus (28:10-19). Jacob, the son of Isaac and grandson of Abraham, was fleeing from his twin brother Esau who had vowed to kill him. On his way to a relative’s house, Jacob lay down for the night. As he was dreaming, he had a vision of a ladder between heaven and earth. God’s angels were on it...

The paper presents the last version of a rad-hard digital chip together with a full end-ladder card prototype of the data acquisition (DAQ) chain of the ALICE SDD experiment. The chip has been designed and constructed using a rad-hard digital library provided by CERN. The end-ladder card includes all the electronic devices foreseen for the DAQ chain of ALICE SDD experiment. Moreover the card in...

In this paper some new results for multidimensional m-band bi-orthogonal lter banks are presented. In the rst part of the paper we introduce the ladder structure as a method for the design and implementation of afore-mentioned lter banks. The second part of the paper fo-cuses upon enforcing linear phase (LP) conditions in the context of ladder structures. The formulation of the LP conditions is...

A systematic approach for the design of Switched Current (SI) ladder filters is described. The specifications of the filter in z-domain are used to obtain continued fraction expansions (CFE). Signal Flow Graphs (SFG) of each row of the expansion is derived and then these SFG are directly transformed into switched current structures (integrators and amplifiers). The SI blocks thus obtained are c...