Chromosomes are replicated in characteristic, temporal patterns during S phase. We have compared the timing of association of replication proteins at early- and late-replicating origins of replication. Minichromosome maintenance proteins assemble simultaneously at early- and late-replicating origins. In contrast, Cdc45p association with late origins is delayed relative to early origins. DNA pol...

Since several years dynorphin, a member of the opioid peptide family, was suggested to play a regulatory role in numerous functional pathways of the brain. In line with its localization in hippocampus, amygdala, hypothalamus and striatum these functions resemble learning and memory, emotional control and stress response. Male prodynorphin-deficient (Dyn KO) mice display an anxiolytic phenotype ...

The reproductive system fully develops during postnatal stages of life, and as such it may be susceptible to environmental cues such as high temperature. Thus, the purpose of the study was to compare how exposure to 34 degrees Celsius ambient temperature affects pituitary-gonadal axis of immature and adult female rats. Three groups of females at proestrous or metaestrous (n=38) were used in the...

Sex-related differences in the perception and modulation of pain have been reported. The present study is the first to investigate systematically whether activation of opioid receptor-like 1 receptor (ORL1) by orphanin FQ (OFQ) produces sex-specific modulation of spinal nociception and whether estrogen or testosterone contributes to these differences using the rat as an experimental animal. Two...

Synthesis of new protein has been shown to be required for establishment of a late phase in hippocampal long-term potentiation. Whether a similar requirement is needed to produce the late phase of long-term depression (LTD) remains to be determined. Application of transcription inhibitors or of the translation inhibitor anisomycin, immediately, but not 30 min after glutamate/depolarization conj...

Suspension cultures of Catharanthus roseus were used to evaluate ultraviolet-B (UV-B) treatment as an abiotic elicitor of secondary metabolites. A dispersed cell suspension culture from C. roseus leaves in late exponential phase and stationary phase were irradiated with UV-B for 5 min. The stationary phase cultures were more responsive to UV-B irradiation than late exponential phase cultures. C...

Leukemia inhibitory factor (LIF) is a pleiotropic IL-6 family cytokine and its maternal uterine expression is critical for mouse blastocyst implantation. In the golden hamster (Mesocricetus auratus), although the blastocyst hatching phenomenon is quite interesting and LIF is shown to regulate hatching, information is not available on the embryonic and uterine expression of LIF and hormonal regu...

To evaluate urinary acidification defect and its contribution to metabolic acidosis (MA) during hemorrhagic fever with renal syndrome (HFRS), we serially analyzed acid-base balance and urinary acidification indices in 10 HFRS patients. Data of the patients were compared with those of 8 normal volunteers (NC). MA was observed in 6 of 8 patients in the oliguric phase, 5 of 7 in the early diuretic...

new simplified tectonic models and depositional history of late cretaceous rocks are established in a part of zagros orogenic belt that is located in the northeastern iraq. these rocks constutes the most important cretaceous oil reservoir in the middle east. the dependent tools are petrography, field study and the concept of drowning phases. this concept is relatively new and accurate in explan...

The novel bicyclo thieno 1, 2, 3-triazines (BTT) namely BTT-1, BTT-2, BTT-3 and BTT-4 were evaluated for analgesic anti-inflammatory and antiarthritic activity. Analgesic and anti-inflammatory activity was evaluated using hot plate test , formalin induced paw licking test and formalin induced paw edema test respectively, complete fruend’s adjuvant (CFA) induced arthritis model was used for anti...