The nature, kinetics and cofactor requirements of leukocyte adhesion to cultured vascular cells have been investigated in vitro, using a model system in which leukocyte suspensions are in continuous motion over the cultured cells. Adhesion is assessed by histological examination or by using 51Cr-labelled leukocytes. Leukocytes adhere preferentially to endothelial cell monolayers rather than to ...

Evidence that the bactericidal ability and the stimulated oxidative metabolism of leukocytes appear in parallel during fetal development of the Minnesota Miniature pig has been obtained by application of the techniques applied to studies of human cells. It was demonstrated that leukocytes from 87- to 90-day fetuses were fully capable of ingesting Staphylococcus aureus but greatly diminished in ...

Diabetic retinopathy is one of the most common diabetic complications, and is a major cause of new blindness in the working-age population of developed countries. Progression of vascular abnormalities, including the selective loss of pericytes, formation of acellular capillaries, thickening of the basement membrane, and increased vascular permeability characterizes early nonproliferative diabet...

ERS ROGER C. CRAFTS, PH.D. , Boston OLIVER P. Jo s, Pn.D., Buffalo CHARLES P. EMERSON, M.D., Boston C. MAIER, M.D. , Zurich, Switzerland SOLOMON ESTREN, M.D., N w YORK JEAN P. S0ULIER, M.D. , Paris ROBERT S. EVANS, M.D. , San FranCisco RAMON M. SUAREZ, San Juan, Puerto Rico CLEMENT A. FINCH, M.D. , Boston JAN WALDEN-STROM, M.D. , Upsala, Sweden

1. Abstract 2. Introduction 3. Discussion 3.1. β2 integrin engagement induces activation of tyrosine kinases 3.1.1 Activation of src family tyrosine kinases 3.1.2. Activation of Syk 3.1.3. Activation of tyrosine kinases of the FAK family 3.2. Proteins that are tyrosine phosphorylated upon β2 integrin engagement in leukocytes 3.2.1. The docking protein Cbl 3.2.2. The guanine nucleotide exchange ...

The expression of acetylcholinesterase in proinflammatory cells has supported the hypothesis that this protein plays a role in intercellular adhesion. Previous results of our group show that velnacrine, an acetylcholinesterase inhibitor, increases the number of adherent leukocytes in post-capillary venules of Wistar rats' mesentery muscle. This works intends to evaluate the local application of...

In their review of the carbohydrate metabolism of leukocytes, Beck and Valentine (4) stated that normal myeloid leukocytes possess a high rate of aerobic glycolysis. Various reports (8, 9, 11) have indicated that, quantitatively, leukocytes from persons with leukemia do not have this capacity to make lactic acid aerobically. However, leukemic leukocytes seem to possess an anaerobic glycolysis, ...

Reperfusion of ischemic tissues results in development of a proinflammatory, prothrombogenic phenotype, culminating in the recruitment of leukocytes and platelets within postcapillary venules. Recent studies have indicated an interdependence of platelet and leukocyte adhesion, suggesting that heterotypic blood cell interactions may account for postischemic platelet recruitment. The objectives o...

Epstein-Barr virus (EBV)-induced transformation effi ciencies were quantitated for peripheral blood leukocytes from two patients with xeroderma pigmentosum (XP) and a patient with the related disorder Cockayne's syndrome. By transformed centers or limiting dilution assays, there were no observed differences in transformation efficien cies between XP, Cockayne's syndrome, or normal adult leukocy...

PURPOSE To evaluate quantitatively leukocyte dynamics in vivo in the rat retinal microcirculation during ischemia reperfusion injury with the use of acridine orange digital fluorography. METHODS Retinal ischemia was induced in anesthetized pigmented rats by a temporary ligation of the optic nerve. After 60 minutes of ischemia, leukocyte behavior in the retinal microcirculation was evaluated, ...