Leydig cells have been found to be either unidentifiable or at apparent low numbers during routine histologic examination of bowhead whale testes. Therefore, formalin-fixed, paraffin-embedded testicular tissues from 14 whales were retrospectively examined determine if immunohistochemical staining could aid in identification Sertoli and cells. Multiple intratesticular samples when available. dif...

Klinefelter's syndrome (47, XXY) is the most common chromosome aneuploidy in men and is usually characterized by underdeveloped testes and sterility. The aim of the present study was to detect cellular distribution of androgen receptors (AR) and aromatase in testes of patient with KS. The tissue sections were processed for morphological and immunohistochemical staining. Additionally, levels of ...

Previous studies have shown that transcription factors GATA-4 and GATA-6 are expressed in granulosa and thecal cells of the mouse ovary and that GATA-4 expression in ovarian tissue is regulated by gonadotropins. Given the emerging role of GATA-4 and GATA-6 in gonadal cells, we have now studied the expression and regulation of these factors in the mouse testis and testicular cell lines. In situ ...

Androgen biosynthesis in males occurs to a large extent in testicular Leydig cells. This study focused on the evaluation of three murine Leydig cell lines as potential screening tool to test xenobiotics interfering with gonadal androgen synthesis. The final step of testosterone (T) production in Leydig cells is catalyzed by the enzyme 17β-hydroxysteroid dehydrogenase 3 (17β-hsd3). The endogenou...

The cellular mechanisms responsible for age-related decline in the ability of Leydig cells to produce testosterone are not yet fully understood. The decline in testosterone production could result from a reduction in the Leydig cell enzymatic activities mediating testosterone synthesis, the amount of substrate available for these enzymes, or both. In the present study, we examined the effect of...

Background: Ceftriaxone is an antibiotic used for treating respiratory,skin,bone and kidney infections. Our purpose was investigating effect of ceftriaxone on testicular tissue. Materials and Methods: 40 mice with 6 weeks old were used in this study. The mice randomly were divided into 2 groups, control- sham and test groups. The test group was subdivided to IP1 and IP2 which received Ceftriaxo...

Received October 2007 Accepted June 2008 INTRODUCTION Leydig cell tumors (LCTs) are the most common stromal tumors, accounting for 3% of all testicular neoplasms.(1) Approximately, 3% of the LCTs are bilateral.(1) They may be hormonally active, leading to either feminizing or virilizing syndromes. About 10% of them are malignant.(1) The diagnosis of a malignant LCT is not always easy, because n...

Objectives The aim of this study was to explore the effects of Squid ink polysaccharide (SIP) on prevention of autophagy and oxidative stress induced by cyclophosphamide (CP) in Leydig cells of mice. Materials and Methods Examination of reproductive organ exponents, abnormal sperm rate, activities of superoxide dismutase (SOD), catalase (CAT), contents of malondialdehyde (MDA), and histologic...

The aim of this study was to evaluate the cellular changes that occur in the hamster testicular interstitium in two very different physiological situations involving testicular involution: ageing and exposure to a short photoperiod. The animals were divided into an 'age group' with three subgroups - young, adult and old animals - and a 'regressed group' with animals subjected to a short photope...

We report the case of an infant who presented at birth with a hypoplastic phallus associated with hypospadias. Low testosterone production, normal serum levels of steroid precursors, and increased LH in response to LH-releasing hormone supported a defect in Leydig cell differentiation or function. Conventional microscopic study of the testes showed fibroblastic cells in the interstitium. Howeve...