نتایج جستجو برای: linked immunospot assay

تعداد نتایج: 428010  

Journal: :iranian red crescent medical journal 0
zahra elahian firouz department of microbiology, islamic azad university, science and research ayatollah amoli branch, amol, ir hami kaboosi department of microbiology, islamic azad university, science and research ayatollah amoli branch, amol, ir abdolreza faghih nasiri department of infectious disease, iran blood transfer organization, sari, ir iran seyed saleh tabatabaie ent-hns research center, hazrate rasool hospital, iran university of medical sciences, tehran, ir iran farideh golhasani-keshtan department of physiology, mashhad university of medical sciences, mashhad, ir iran fatemeh zaboli department of mycology, islamic azad university, science and research ayatollah amoli branch, amol, ir iran; department of mycology, islamic azad university, science and research ayatollah amoli branch, amol, ir iran. tel: +98-1212517001, fax: +98-1212517001

results: of the 50 samples tested by the elisa method, 26 samples (52%) were positive for igg . no samples were positive for igm. of the 50 samples tested by the chemiluminescence method, 28 samples (56%) were positive for igg. no samples were positive for igm. conclusions: a significant relationship between the age of the youngest child and the infection rate was seen. no significant correlati...

2017
Steven G Smith Stephanie A Harris Iman Satti Donna Bryan K Barry Walker Hazel M Dockrell Helen McShane Mei Mei Ho

Cellular immunological assays are important tools for the monitoring of responses to T-cell-inducing vaccine candidates. As these bioassays are often technically complex and require considerable experience, careful technology transfer between laboratories is critical if high quality, reproducible data that allows comparison between sites, is to be generated. The aim of this study, funded by the...

Journal: :Acta medica Okayama 2014
Ryuichiro Tsuzaki Akinobu Takaki Takahito Yagi Fusao Ikeda Kazuko Koike Yoshiaki Iwasaki Hidenori Shiraha Yasuhiro Miyake Hiroshi Sadamori Susumu Shinoura Yuzo Umeda Ryuichi Yoshida Daisuke Nobuoka Masashi Utsumi Eiichi Nakayama Toshiyoshi Fujiwara Kazuhide Yamamoto

It is not known how the immune system targets hepatitis C virus (HCV)-infected HLA-mismatched hepatocytes under immune-suppressed conditions after orthotopic liver transplantation (OLT). In addition, the relationship between the HCV-specific immune response and IL28B variants as predictors of HCV clearance has not been well-characterized. We determined the IL28B polymorphisms for 57 post-OLT HC...

2015
Dumitru Chesov Christoph Lange Franziska Daduna Valeriu Crudu Rosemarie Preyer Martin Ernst Barbara Kalsdorf

BACKGROUND To evaluate interleukin (IL)-2 and interferon (IFN)-γ secreting T-cells in parallel for the differentiation of latent infection with Mycobacterium tuberculosis infection (LTBI) from active tuberculosis. METHODS Following ex-vivo stimulation of peripheral blood mononuclear cells (PBMC) with M. tuberculosis-specific antigens early secretory antigenic target (ESAT)-6 and culture filtr...

2013
Sandra A. Calarota Fausto Baldanti

The enzyme-linked immunospot (ELISPOT) assay has advanced into a useful and widely applicable tool for the evaluation of T-cell responses in both humans and animal models of diseases and/or vaccine candidates. Using synthetic peptides (either individually or as overlapping peptide mixtures) or whole antigens, total lymphocyte or isolated T-cell subset responses can be assessed either after shor...

Journal: :Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 2009
Douglas J Begg Kumudika de Silva Katrina Bosward Lyrissa Di Fiore Deborah L Taylor Gregers Jungersen Richard J Whittington

To date, the sensitivity of the interferon gamma (IFN-gamma) enzyme-linked immunosorbent assay (ELISA) to detect Johne's disease (JD) has been poor, especially in the early stages of disease. To improve the sensitivity of IFN-gamma detection in the early stages of infection, an alternate assay needs to be developed. The enzyme-linked immunospot (ELISPOT) assay is a highly sensitive technique fo...

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