Lipoprotein lipase (LPL), the rate-limiting enzyme for hydrolysis of plasma lipoprotein triglycerides, is a normal constituent of the arterial wall. We explored whether LPL affects (a) lipoprotein transport across bovine aortic endothelial cells or (b) lipoprotein binding to subendothelial cell matrix (retention). When bovine milk LPL was added to endothelial cell monolayers before addition of ...

MALLOV, SAMUEL, AND ADAWIA A. ALOUSI. Effect of altered cardiac metabolism and work on lipoprotein lipase activity in heart. Am. J. Physiol. 2 I 2 (5) : I I 58-1 I 64. I g67.-Increased cardiac metabolism and/or work were induced in rats by: a) chronic administration of 2 ,4-dinitrophenol, b) aortic constriction, and c) production of hypertension with deoxicorticosterone (DCA) and salt. Decrease...

The mechanisms following intimal injury predisposing towards atherosclerotic changes have not been fully elucidated. We speculated that a local increase in the enzyme lipoprotein lipase (LPL) might explain a higher susceptibility of the damaged intima to lipid accretion, and so we investigated the effect of balloon endothelial denudation on LPL activity and cholesterol content (LPLa and Cholc, ...

Endothelial lipase (EL) is a member of a subfamily of lipases that act on triglycerides and phospholipids in plasma lipoproteins, which also includes lipoprotein lipase and hepatic lipase. EL has a tropism for high density lipoprotein, and its level of phospholipase activity is similar to its level of triglyceride lipase activity. Inhibition or loss-of-function of EL in mice results in an incre...

To test the hypothesis that hepatic lipase plays a key role in lipoprotein removal in vivo, a novel system was used. Hepatoma cells (HTC 7288c) were transfected with a cDNA encoding hepatic lipase in culture and grown as solid tumors in vivo. In culture, transfected cells degraded chylomicron remnants and low density lipoprotein (LDL) somewhat more efficiently than untransfected cells. Tumors f...

Lipoprotein lipase (LPL) from rat heart acetone powders has been reported to depend on the presence of NH4 + , calcium, or other divalent cations for optimal activity. In addition, the enzyme will not hydrolyze an artificial triglyceride emulsion unless it is converted to an active substrate by the addition of very low density lipoproteins, high density lipoproteins (HDL), or certain peptides c...

Lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) enhance the hydrolysis of triglycerides (TG) transported by chylomicron (CM) and very-low-density lipoprotein (VLDL). We report a case of severe hyperchylomicronemia with high levels of remnant lipoprotein and total cholesterol (T-Chol) in a 15-year-old boy. Precise examination of the lipid profile showed decreased activities of bo...

Recent studies have indicated that the activity of lipoprotein lipase in rat adipose tissue is related to the nutritional state of the animal. Fasting induces a fall in the activity released from surviving tissue ly heparin and in the activity of homogenates of adipose tissue, while glucose and insulin in vitro increase the heparin effect on the release of lipase from surviving adipose tissue o...

S U M M A R Y A method has been described for the quantitative assay of lipoprotein lipase activity in post heparin plasma. The conditions of the away were subjected to critical study. The technique described permits the react.ion to proceed in the test tube as a zero order reaction. It is believed that this technique will permit accurate quantitative study of lipoprotein lipase activity in dif...