Bacterial lipoproteins are lipid-anchored proteins that contain acyl groups covalently attached to the N-terminal cysteine residue of the mature protein. Lipoproteins are synthesized in precursor form with an N-terminal signal sequence (SS) that targets translocation across the cytoplasmic or inner membrane (IM). Lipid modification and SS processing take place at the periplasmic face of the IM....

Monolayers of cultured fibroblasts from normal human subjects bind (125)I-labeled low-density lipoproteins with high affinity and specificity. High affinity binding of similar magnitude was not observed in cells from five unrelated subjects with the homozygous form of familial hypercholesterolemia. In normal cells incubated at 37 degrees , the binding sites were saturated at a low-density lipop...

found in the response of the electron-capture detector for fatty acids of niicrosomal lipids from CC1,-treated rats (the latter peak is clearly a group of unresolved peaks). Since serum very-low-density lipoproteins derive directly from the hepatic cell and since the phospholipid fraction of such lipoproteins is believed to derive from the phospholipids of the endoplasmic reticulum, we investig...

Apoproteins from plasma lipoproteins were localized by immunofluorescence techniques in human carotid artery atherosclerotic lesions. These studies were performed in light of the possible importance of these apoproteins in both lipid metabolism and the pathogenesis of atherosclerosis. ApoA-I from high density lipoproteins, apoB from low density lipoproteins, and apoC-III from very low density l...

We tested the hypothesis that apolipoproteins, the protein constituents of plasma lipoproteins, are secreted into bile. We examined human gallbladder bile obtained at surgery (N = 54) from subjects with (N = 44) and without (N = 10) gallstones and hepatic bile collected by T-tube drainage (N = 9) after cholecystectomy. Using specific radioimmunoassays for human apolipoproteins A-I and A-II, the...

Smooth muscle cells (SMC) were grown from inner media explants of swine aorta and used as a model for studying the role of lipoproteins in atherogenesis. These cultured cells retain the characteristics of SMC through multiple passages. Cell growth curves, in time, were obtained by using standard counting techniques, SMC grew slowly (0.019 cycle/day) in modified Dulbecco-Vogt medium supplemented...

In Gram-negative bacteria, outer membrane-associated lipoproteins can either face the periplasm or protrude out of the bacterial surface. The mechanisms involved in lipoprotein transport through the outer membrane are not fully elucidated. Some lipoproteins reach the surface by using species-specific transport machinery. By contrast, a still poorly characterized group of lipoproteins appears to...

OBJECTIVE The effect of n-3 polyunsaturated fatty acids on the serum lipid profile in a Mexican population was evaluated. MATERIAL AND METHODS Three g of salmon oil was the daily intake during four weeks. Total cholesterol, triglycerides, low density lipoproteins, high density lipoproteins and erythrocyte fatty acid composition were analyzed. RESULTS The hypertriglyceridemic group showed a ...

Apolipoprotein B is the main structural surface protein found on all beta-lipoproteins (Chylomicrons, VLDLs, IDLs and LDLs). There is a single molecule of apoB on each of those lipoproteins. It is the only apolipoprotein that is not transferablei.e. it is with the particle from its birth till its death. Beta-lipoproteins are the lipoproteins capable of trafficking cholesterol into the artery wa...

High serum cholesterol is a major risk factor for coronary heart disease (CHD), and most cholesterol in serum circulates with low density lipoproteins (LDLs). These lipoproteins are macromolecular complexes containing a core of nonpolar lipids — mainly cholesterol ester—and a surface coat of unesterified cholesterol, phospholipids, and apolipoprotein (apo) B-100. Many investigators believe that...