In this work, the application of multivariate curve resolution-alternating least squares (MCR-ALS) is proposed for extracting information from multitechnique fused multivariate data (UV-VIS absorption, fluorescence, and liquid chromatography-mass spectrometry) gathered during the biosynthesis of violacein pigment. Experimental data sets were pretreated and arranged in a row-wise augmented data ...

The continuous-flow fast atom bombardment probe performs equally well with or without a high-performance liquid chromatography column producing clean spectra containing little or no background noise. Its function as a liquid chromatography-mass spectrometry interface for labile and involatile samples has been illustrated with reference to dansylated amino acids. The versatility of the new probe...

In forensic analysis of explosives, analytical methods are required that combine good separation characteristics with highly specific and sensitive detection. Two novel techniques, liquid chromatography/mass spectrometry (LC/MS) and tandem mass spectrometry (MS/MS) have such specifications. These are now well-established analytical techniques for separation and identification of mixtures and fo...

A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed and validated for the determination of donepezil in human plasma samples. Diphenhydramine was used as the internal standard. The collision-induced transition m/z 380 --> 91 was used to analyze donepezil in selected reaction monitoring mode. The signal intensity of the m/z 380 --> 91 transition was found to relate l...

Traditional analysis of liquid chromatography-mass spectrometry (LC-MS) data, typically performed by reviewing chromatograms and the corresponding mass spectra, is both time-consuming and difficult. Detailed data analysis is therefore often omitted in proteomics applications. When analysing multiple proteomics samples, it is usually only the final list of identified proteins that is reviewed. T...

Discovery of protein biomarkers in clinical samples necessitates significant prefractionation prior to liquid chromatography-mass spectrometry (LC-MS) analysis. Integrating traveling wave ion mobility spectrometry (TWIMS) enables in-line gas phase separation which when coupled with nanoflow liquid chromatography and data independent acquisition tandem mass spectrometry, confers significant adva...

The rapid identification of novel plant metabolites and assignments of newly discovered substances to natural product classes present the main bottlenecks to defining plant specialized phenotypes. Although mass spectrometry provides powerful support for metabolite discovery by measuring molecular masses, ambiguities in elemental formulas often fail to reveal the biosynthetic origins of speciali...

New analytical methods for the determination of pesticide residues in wine were introduced and validated. Extraction was performed with sorption active substances on stir bar by gas chromatography-mass spectrometry liquid chromatography-tandem mass spectrometry. The applied practice. A total 77 searched 42 samples from all 3 wine-growing regions Slovenia. fungicides dimethomorph, fludioxonil py...

1 Abbreviations used: [methyl-C]AdoMet, S-adenosyl-L-[methyl-C]methionine; [methyl-H]AdoMet, S-adenosyl-L-[methyl-H]methionine; aDMA, asymmetric x-N,N-dimethylarginine; AdoMet, S-adenosyl-L-methionine; AdoHcy, S-adenosylL-homocysteine; CID, collision-induced dissociation; DMA, dimethylarginine; DTT, dithiothreitol; ESI, electrospray ionization; ETD, electron-transfer dissociation; FPLC, fast pe...

The high performance liquid chromatography-electrospray ionization-mass spectrometry method has been applied to the measurement of bile acid transport activities in membrane vesicles obtained from a human bile salt export pump expressing Sf9 cells. The amounts of bile acids transported using the human bile salt export pump expressing Sf9 cells were determined using liquid chromatography-electro...