The exposure of HL-60 human promyelocytic leukemia cells to 0.5 microM 5-fluoro-2'-[3H]deoxyuridine (FdUrd) for 16 hr resulted in the incorporation of 5.14 +/- 0.31 (S.D.) X 10(-7) mol FdUrd into DNA per mol of DNA nucleotide, which corresponds to 0.146 +/- 0.082 pmol FdUrd per 10(7) cells. Pretreatment with 50 microM deoxythymidine for 24 hr led to a 2.7-fold increase in the incorporation of t...

background and objectives: a single reactive igg anti-dengue virus elisa test in the absence of igm antibodies or ns1 antigen may denote current infection or past exposure to the virus. to determine whether igg index value can be used to identify true current dengue infection we conducted a prospective observational study. materials and methods: suspected dengue patients (n =1745) were tested i...

Cigarette smoke exposure has been associated with a variety of diseases, including emphysema. The current study evaluated the interaction of cell density and cigarette smoke extract (CSE) on fibroblast contraction of collagen gels. Protein levels of transforming growth factor (TGF)-beta1, fibronectin, PGE(2), and TGF-beta1 mRNA were quantified. Although both 5 and 10% CSE inhibited contraction ...

In polytene chromosomes of Drosophila hydei and D. melanogaster, Z-DNA was identified in varying distribution after different conditions for fixation were used. When salivary glands were fixed and squashed in 50% acetic acid alone, Z-DNA was found in the less dense DNA regions, such as interbands, some puffs, and a few of the less dense bands. Prefixation that combined ethanol and acetic acid e...

OBJECTIVE Acrolein is a toxic chemical present in tobacco, wood, and coal smoke, as well as automobile exhaust. Because exposure to these pollutants is associated with an increase in cardiovascular disease risk, we studied the effects of acrolein on Flk-1(+)/Sca-1(+) cells that are involved in vascular repair. METHODS AND RESULTS In adult male C57BL/6 mice, inhalation of acrolein (1 part per ...

Introduction Volatile organic compounds (VOCs) tend to be liquids which readily give off vapour at room temperature1, such as solvents and fuels. At high concentrations, these vapours can explode. At extremely low levels, they can be toxic. While the impact of exposure can sometimes be felt immediately, frequently symptoms may not become apparent for months if not years afterwards. This type of...

introduction: low level laser therapy (lllt) also known as photobiomodulation, is a treatment that uses low-level lasers or light-emitting diodes (leds) to change cellular function and is a clinically well accepted tool in regenerative medicine and dentistry. considering the variety of laser, exposure, cells and study types, the exact effects of low level laser therapy seems to be unclear. the ...