Protein post-translational modifications (PTMs) at the lysine residue, such as lysine methylation, acetylation, and ubiquitination, are diverse, abundant, and dynamic. They play a key role in the regulation of diverse cellular physiology. Here we report discovery of a new type of lysine PTM, lysine malonylation (Kmal). Kmal was initially detected by mass spectrometry and protein sequence-databa...

S-Adenosylmethionine (SAM) and an iron-sulphur centre function in place of adenosylcobalamin. Because of its coenzyme BJike role in this reaction, SAM was originally described by H. A. Barker as ‘A poor man’s adenosylcobalamin’ [4]. The conversion of lysine into P-lysine by lysine 2,3-aminomutase proceeds without exchange of solvent protons with substrate hydrogen, and by the stereochemistry il...

The carboxylation of lysine residues is a post-translational modification (PTM) that plays a critical role in the catalytic mechanisms of several important enzymes. It occurs spontaneously under certain physicochemical conditions, but is difficult to detect experimentally. Its full impact is unknown. In this work, the signature microenvironment of lysine-carboxylation sites has been characteriz...

1. The kinetics of 2-min uptake of L-lysine and L-lysyl-L-lysine have been studied by using rings of everted hamster intestine in vitro, and values for Kt and Vmax. established. 2. On a molar basis, mediated uptake was more rapid for the amino acid than for the peptide. Non-mediated uptake was more rapid for the peptide than for the amino acid. 3. Comparison of relative rates of uptake of lysin...

Recently, we reported the lysine requirement to be 41.2 mg.kg-1.d-1 using indicator amino acid oxidation (IAAO) in healthy adult males fed a protein intake of 1.0 g.kg-1.d-1. A lower protein intake has been hypothesized to significantly lower the requirement estimate. We tested this hypothesis using IAAO by estimating the lysine requirement at a protein intake of 0.8 g.kg-1.d-1 in five healthy ...

We recently reported the lysine requirement of school-aged children living in Canada consuming a mixed diet to be 35 mg.kg(-1).d(-1). Because the majority of children in the world live on cereal-based diets in developing countries, we measured the daily lysine requirement in healthy children living in India and consuming a mostly cereal-based diet. Our objective in this study was to determine t...

Two naturally selected microbial populations were maintained under continuousflow conditions with glucose or magnesium growth-limiting. The reactors were subjected to shock loads by changing the influent substrate from L-lysine to a mixture of L-lysine and glucose, L-lysine and fructose, or L-lysine and ribose. During the subsequent transient state, the following parameters were examined: lysin...

The aim of this study was to investigate the antibacterial characteristics and antibacterial mechanisms of 3-poly-lysine against Escherichia coli and Staphylococcus aureus. The diameters of inhibition zones of E. coli (10 0.5 mm) and S. aureus (12 0.1 mm) treated by 200 mg/ml 3-poly-lysine were much larger than control (5 0.3 mm) (p < 0.05). Minimum inhibition concentration of 3-poly-lysine aga...