The hypothesis that C60 fullerene nanoparticles (C60) exert an antagonistic interactive effect on the toxicity of benzo[a]pyrene (BaP) has been supported by this investigation. Mussels were exposed to BaP (5, 50 & 100 ?g/L) and (C60–1 mg/L) separately in combination. Both shown co-localize secondary lysosomes hepatopancreatic digestive cells gland where they reduced lysosomal membrane stability...

Graphene-based nanomaterials (GNMs), including graphene, graphene oxide, reduced and quantum dots, may have direct anticancer activity or be used as nanocarriers for antitumor drugs. GNMs usually enter tumor cells by endocytosis can accumulate in lysosomes. This accumulation prevents drugs bound to from reaching their targets, suppressing effects. A number of chemical modifications are made fac...

The biochemistry and intracellular transit of an integral membrane glycoprotein of chicken fibroblast lysosomes were studied with monoclonal antibody techniques. The glycoprotein had an apparent molecular weight of 95,000-105,000. Structural analysis involving metabolic labeling with [35S]methionine and cleavage with glycosidases revealed the presence of numerous oligosaccharide chains N-linked...

to determine the effect of different levels of l-glutamine and glycerol on freezing coated sperm, semen were collected from 4 taleshi rams through artificial vagina contact with a tube containing tris-fructose-egg yolk 15%. ejaculates were pooled, centrifuged and supernatant was removed, then cell fractions were used. samples were split into 9 equal parts and 3 (g3), 5 (g5) or 7% (g7) glycerol ...

Neurite outgrowth is mediated by the exocytosis of intracellular vesicles at the tips of elongating neuronal processes. The lysosomal vesicle-associated soluble N-ethylmaleimide-sensitive factor attachment protein receptor tetanus neurotoxin insensitive vesicle-associated membrane protein (TI-VAMP)/VAMP7 was previously implicated in membrane fusion events mediating neurite outgrowth, but the pa...

Gentamicin, an aminoglycoside antibiotic used to treat severe bacterial infections, may cause acute renal failure. At therapeutic concentrations, gentamicin accumulates in lysosomes and induces apoptosis in kidney proximal tubular cells. In gentamicin-treated renal LLC-PK1 cells, acridine orange release from lysosomes, previously interpreted as lysosomal membrane permeabilization, precedes the ...