AVR1674 and AVR1675 are monoclonal antibodies (mAbs) that bind with high specificity to anthrax toxin lethal factor (LF) and lethal toxin (LTx). These mAbs have been used as pivotal reagents to develop anthrax toxin detection tests using mass spectrometry. The mAbs were demonstrated to bind LF with good affinity (KD 10−7–10−9 M) and to enhance LF-mediated cleavage of synthetic peptide substrate...

Recently it was discovered that the bacteriophage M13 contains in its protein III gene, tandem repeats of a 15-bp motif that can recognize a family of hypervariable minisatellites in vertebrate DNA (1). Southern blots of human DNA digested with Hae III and probed with 32p_iaDeled M13 revealed complex individual-specific DNA fingerprints, similar to those described by Jeffreys et al (2) with pro...

Recombinant baculoviruses offer a useful way of expressing eucaryotic genes, often in high yield (1). Current procedures for the generation of a recombinant virus make use of a transfer vector that, via recombination in vivo, place the gene of interest under control of the powerful polyhedrin promotor. At present the expression of mutant forms of the cloned gene (to study structure and function...

F-specific filamentous phage of Escherichia coli (Ff: f1, M13, or fd) are long thin filaments (860 nm × 6 nm). They have been a major workhorse in display technologies and bionanotechnology; however, some applications are limited by the high length-to-diameter ratio of Ff. Furthermore, use of functionalized Ff outside of laboratory containment is in part hampered by the fact that they are genet...

Toxoplasma gondii is a ubiquitous, unicellular, eukaryotic parasite with a complex intracellular life cycle capable of invading and chronically infecting a wide variety of vertebrate host species, including man. Although normally opportunistic in healthy adults, it is a lethal pathogen in immunocompromised humans, particularly in AIDS patients. We present the application of a genomic phage disp...

In growing cells infected by wild-type coliphage M13, the synthesis of procoat protein is completed before it inserts into the plasma membrane ane is converted to coat protein.

eindelijk die kast opruimen!). Summary 108 Samenvatting 111 Curriculum vitae 115 List of Publications 117 Het in dit proefschrift beschreven onderzoek werd mogelijk gemaakt met financiële steun van de Stichting Levenswetenschappen (SLW) van de Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO). Abbreviations ATR-FTIR attenuated total reflection Fourier transform infrared CD circular ...