Pyruvate dehydrogenase (PDHb) phosphatase has been purified to apparent homogeneity from mitochondria of the adult parasitic nematode, Ascaris suum. The enzyme is a heterodimer of 89 and 50 kDa, as judged by SDS-polyacrylamide gel electrophoresis. It appeared to copurify with its substrate, pyruvate dehydrogenase (E1) and could be separated by chromatography on Superose 12 in the presence of 0....

Soluble-protein and eight enzyme profiles obtained by polyacrylamide-gel electrophoresis were compared between Meloidogyne incognita and M. arenaria. Esterase, malate dehydrogenase, and alpha-glycerophosphate dehydrogenase patterns were distinctly characteristic for each species. Peroxidase and alpha-glycerophosphate dehydrogenase isoenzyme patterns varied when nematodes were propagated on diff...