Bacillus subtilis HM1 was isolated from the rhizosphere region of halophytes for its antifungal activity against Colletotrichum acutatum, the causative agent of anthracnose. Treatment of postharvest apples with the cell culture or with a cell-free culture supernatant reduced disease severity 80.7% and 69.4%, respectively. Both treatments also exhibited antifungal activity against various phytop...

Surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOF-MS) using nanoparticles (NPs) and nanostructured surfaces as the LDI-assisting nanomaterials is a soft ionization technique that features minimal fragmentation of analytes. As compared to traditional matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) using organic ma...

BACKGROUND In Africa, relapsing fever borreliae are neglected vector-borne pathogens that cause mild to deadly septicemia and miscarriage. Screening vectors for the presence of borreliae currently requires technically demanding, time- and resource-consuming molecular methods. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has recently emerged as a to...

We present the first application of the quality threshold (QT) clustering algorithm to mass spectrometry (MS) data. The unique abilities of QT clustering to yield precision nodes that are commensurate with the mass measurement precision of the instrument are exploited to generate a consensus spectrum out of multiple replicate spectra. The spectral dot product and confidence intervals are used a...

background: transforming growth factor-beta (tgf-β) activation appears to be crucial for tissue injury in diabetic nephropathy (dn). fibromodulin, the small leucine-rich proteoglycan, has been proposed to be the potent tgf-β modulator. in this study, the therapeutic effects of fibromodulin in the kidneys of streptozotocin (stz)-induced diabetic rats were investigated. methods: diabetic rats rec...

Identification of individual proteins in complex protein mixtures by high-resolution (HR), high-mass-accuracy matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry (TOF-MS) is demonstrated for synthetic protein mixtures. Instead of chemical denaturation, thermal denaturation followed by in-solution trypsin digestion is used to achieve uniform digestion of the cons...

We studied the possibility of detection [Ru(η5-C5H5)(PPh3)2Cl] (abbreviated by RuCp) complex as a model system for Ru-based metallodrugs in human urine using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) without previous purification or removal inorganic salts. Inorganic salts might prevent RuCp MALDI-TOF MS, most likely through increased number and...

Metabolic exchange between an organism and the environment, including interactions with neighboring organisms, is important for processes of organismal development. Here we develop and use thin-layer agar natural product MALDI-TOF imaging mass spectrometry of intact bacterial colonies grown on top of the MALDI target plate to study an interaction between two species of bacteria and provide dire...

■ SPECTROPHOTOMETRY AND PHOTOMETRY Beer’s Law Spectrophotometric Instruments Components of a Spectrophotometer Spectrophotometer Quality Assurance Atomic Absorption Spectrophotometer Flame Photometry Fluorometry Chemiluminescence Turbidity and Nephelometry Laser Applications ■ ELECTROCHEMISTRY Galvanic and Electrolytic Cells Half-Cells Ion-Selective Electrodes pH Electrodes Gas-Sensing Electrod...

Sample pretreatment was optimized to obtain high sequence coverage for human serum albumin (HSA, 66.5 kDa) when using nano-electrospray ionization quadrupole time-of-flight mass spectrometry (nESI-Q-TOF-MS). Use of the final method with trypsin, Lys-C, and Glu-C digests gave a combined coverage of 98.8%. The addition of peptide fractionation resulted in 99.7% coverage. These results were compar...