A skeletal examination of fetuses is required in regulatory embryo-fetal development studies. This chapter describes a method of skeletal examination using alizarin staining. All fetuses are removed from the mother by caesarean section before birth. The fetuses are first examined externally. For larger species (rabbit and minipig), an internal examination can be performed on the fresh soft tiss...

In developing countries pulmonary tuberculosis is usually diagnosed by detecting acid-fast bacilli (AFB) in sputum using a Ziehl-Neelsen (Z-N) staining method. However, in the field the traditional method of staining is difficult to carry out. This study evaluates the efficiency of two cold staining methods, namely Gabbet’s and a modified two reagent cold staining method compared with the Z-N t...

Epon epoxy resin was introduced as an embedding medium for biological electron microscopy largely through the independent efforts of Finck (1) and Luft (2). The usual recipe involves three components: Epon 812 resin (Epikote 812, in England), an anhydride hardener, and an accelerator such as DMP-30. Since the combination of Epon with dodecenyl succinic anhydride (DDSA) was thought to produce a ...

An automated single-slide staining device is described. Smears gram stained by the automatic device were equal in quality to the manual method.

The rapid fluorescent-antibody staining technique described by Kellogg and Deacon for staining Neisseria gonorrhoeae and Treponema pallidum was applied to fluorescent-antibody tests for group A streptococci and enteropathogenic Escherichia coli. Results obtained with this staining technique were compared with results using the conventional staining procedure; excellent correlation was obtained....

The method of tyramine amplification is based on the ability of HRP to catalyze the deposition of large amounts of biotinylated tyramine in the vicinity of the antigen-antibody reaction (i.e. the cellular site which contains the peroxidase detection complex). The method is based on earlier biochemical observations on the action of peroxidase upon tyramine and other related compounds (GROSS AJ a...