background & objectives : different energy substrates and nutrients can greatly influence oocyte nuclear and cytoplasmic maturation. effect of glucose on in-vitro oocyte maturation and subsequent early embryonic development is controversial. the objective of the present study was to verify the effect of various concentrations of glucose on cumulus-oocyte complexes maturation, fertilization and ...

Background: The maturation medium for in-vitro oocyte maturation is usually supplemented with serum. However, supplementation of serum from pregnant women adversely affects the outcome of in-vitro maturation. The purpose of the study was to assess if growth factors or granulosa cell coculture could overcome the adverse effects of pregnant women’s serum. Materials and Methods: The basal maturati...

this study was designed to clarify bilaterally effect of long-time varicocele on left and right testes and to identify the effect of varicocele on maturation divisions during spermatogenesis. moreover, the percentage of seminiferous tubules (sts) with i-viii and ix-xiv stages of spermatogenesis and sperm parameters such as sperm motility and abnormality types were evaluated. the left varicocele...

in vitro maturation (ivm) is a method that immature oocytes in antral follicles are extracted and matured in laboratry conditions. this review has attempted to provide the current knowledge and recent findings in in vitro maturation of oocytes and highlights the most important factors involved in this process. the review is based on literature reports and the author’s experience. in ivm cycles,...

BACKGROUND The Asian seabass (Lates calcarifer) is a protandrous hermaphrodite that typically matures as a male at approximately 2-4 years of age and then changes sex in subsequent years. Although several sexual maturation stages have been described histologically for both testis and ovary, the underlying gene expression profiles remain lacking. The development of a gene expression platform is ...

We compared the nuclear maturation status and gene-expression profiles of canine cumulus cells (CCs) derived from cumulus-oocyte complexes (COCs) that were spontaneously ovulated versus those that were matured in vitro. Cumulus-oocyte complexes were retrieved from uteri by surgical flushing (after spontaneous ovulation) or by ovariectomy follicle aspiration and in vitro maturation. The objectiv...