Fluted pumpkin seeds were processed into the raw, boiled, fermented, germinated and roasted seeds, dried at 500C, milled and sieved. The seed flours were analyzed for nutritional composition, energy, amino acids and fatty acids of the oils. Processing affected the levels of nutrients in the seed. The energy values ranged between 26.55 ± 0.7 – 30.06 ± 0.8 KJ/g and the seed is a good source of so...

Chemical composition and protein quality of raw and heat-processed (boiled and autoclaved) samples of selected advanced breeding lines of lima beans (Tpl 1, Tpl 7 and Tpl 175), part of a larger collection developed by the International Institute for Tropical Agriculture (IITA), and evaluated in agronomic field trials in Nigeria, were studied. Protein quality was evaluated using weanling albino ...

Two-dimensional TLC analysis of 32P-labeled nucleotides extracted from the yeast Saccharomyces cerevisiae reveals that two highly phosphorylated nucleotides are synthesized during sporulation. These nucleotides have been identified as adenosine 5'-tetraphosphate (ppppA) and adenosine 5'-pentaphosphate (pppppA). The synthesis of ppppA and pppppA commences late in sporulation and follows formatio...

1. Proteins extracted from the seeds of the Euphorbiaceae Croton tiglium and Jatropha curcas were separated into three major peaks (I, Hand III) by Sephadex chromatography. 2. The crude protein from both seeds and peaks I and II from Croton and peak I from Jatropha were toxic to mice, to different extents. 3. The crude protein and peak I and peak II from both seeds, inhibited protein synthesis ...

The pseudotemperate phage PMB12 was isolated from soil on the basis of its ability to enhance the rate of sporulation of Bacillus subtilis 168. PMB12 was subsequently shown to convert the sporulation defect in two genetically distinct classes of sporulation mutants. One class includes those rifampin-resistant mutants that are also spore-negative (mutated at the rif locus). The other class inclu...

BACKGROUND Sporulation of Clostridium difficile during infection and persistence of spores within the gut could partly explain treatment failures and recurrence. However, the influence of antibiotics on sporulation is unclear. The objective of our study was to evaluate the impact of ciprofloxacin, metronidazole, piperacillin/tazobactam, tigecycline, and vancomycin on C. difficile sporulation in...

Clostridium difficile remains a leading nosocomial pathogen, putting considerable strain on the healthcare system. The ability to form endospores, highly resistant to environmental insults, is key to its persistence and transmission. However, important differences exist between the sporulation pathways of C. difficile and the model Gram-positive organism Bacillus subtilis. Amongst the challenge...

Clostridium welchii type A is a common agent of food poisoning when allowed to proliferate to large numbers in cooked foods, usually meat and poultry. The main factors of importance are survival of the spores, frequently found on raw products, through the cooking process, and possible contamination of cooked meats transferred to unclean containers; subsequent germination of spores and rapid mul...

Digestive physiology and movement patterns of animal dispersers determine deposition patterns for endozoochorously dispersed seeds. We combined data from feeding trials, germination tests, and GPS telemetry of Asian elephants (Elephas maximus) to (1) describe the spatial scale at which Asian elephants disperse seeds; (2) assess whether seasonal differences in diet composition and ranging behavi...

OBJECTIVE The purpose was to monitor implanted seeds and to determine factors contributing to seed migration after permanent prostate brachytherapy. METHODS Sixty-two consecutive patients with Stage 1 prostate cancer who underwent brachytherapy with (125)I seeds between February 2008 and May 2009 were studied prospectively. On post-operative days 1, 7 and 30, scintigraphy was added to convent...