A rapid method for the isolation and high-performance liquid chromatographic (HPLC) determination of sulfamethazine (SMZ) in pork tissues (kidney, liver, and muscle) without using organic solvents is developed. The isolation is performed by homogenization with an acid solution using an ultrasonic-homogenizer, followed by centrifugation. The HPLC analyses are performed using a reversed-phase C(4...

Crossflow membrane filtration was used to process recombinant Escherichia coli cell lysates containing protein inclusion bodies after high pressure homogenization. The number of passes through the high pressure homogenizer changed the viscosities and average particle sizes of the cell lysates. The different cell lysates were processed with a hollow fiber unit containing microfiltration membrane...

A procedure is described for isolating cell membranes from rat liver homogenates. 20 gm. of rat liver was homogenized in a Dounce homogenizer in ice cold water buffered to pH 7.5 with NaHCO(3), rupturing all of the cells and most nuclei. The diluted homogenate was filtered through cheesecloth to remove precipitated nucleoprotein and centrifuged at 1500 g, 10 minutes, to sediment a crude membran...

91 in an alkaline Polycell solution for at least 24 h before analysis. When these steps are taken blockage of the manifold occurs infrequently. E excretion in man is predominantly urinary; about 10% of the intake is excreted in the faeces. T h e main source of error in a N balance study is in the estimation of dietary N. Because of the mixed nature of the diet it is difficult to obtain a truly ...

The constructive biology and the synthetic biology approach to creating artificial life involve the bottom-up assembly of biological or nonbiological materials. Such approaches have received considerable attention in research on the boundary between living and nonliving matter and have been used to construct artificial cells over the past two decades. In particular, Giant Vesicles (GVs) have of...

Functional plasma membranes from the filamentous fungus Penicillium chrysogenum have been isolated with the objective of studying transport processes. The isolation procedure consists of three steps, namely homogenization of cells with a Braun MSK homogenizer, followed by Percoll gradient centrifugation and floatation of membranes in a three-step Nycodenz gradient. This method can be applied to...

Conventional solar-pumped lasers rely on expensive and highly accurate solar tracking systems, which present a significant economic barrier to both laser research practical applications. To address this challenge, an end-side-pumped four-rod head was designed built for testing at PROMES-CNRS. Solar radiation collected concentrated using heliostat–parabolic mirror system. A fused silica aspheric...

Cellulose micro/nanofibril (CMNF) as a reinforcing material for composites is becoming more and more attractive to researchers in composite science because of its potential lightweight and high strength. CMNF is generally prepared by either physical treatment, e.g., by a high pressure homogenizer and/or high pressure refiner, or chemical treatments, e.g., acid hydrolysis. In this study, attempt...

Propolis is strong adhesive material with one of most valuable bees product and has wide range of biological activities such as antibacterials, antiviruses, antifungals, antiinflammation, photoprotector and so on. Propolis has been aplied as antimicrobials agent in food and healtcare products. To improve handling properties, Indonesian propolis were encapsulated by casein micelle with a homogen...