In order to overcome limitations of conventional methods, premix membrane emulsification (ME) using Shirasu porous glass (SPG) membranes was investigated for the preparation O/W food-grade nanoemulsions. this study, effects major process variables, formulation and parameters on mean droplet diameter polydispersity (PDI) were systematically investigated. Stability curcumin-loaded nanoemulsions w...

1. A sensitive and specific radioreceptor assay for measuring prolactin in tissue extracts, culture media and serum has been developed utilizing membrane fractions prepared from either chicken or turkey kidney, and an ovine prolactin standard and tracer. 2. Assay sensitivity was 1.0 +/- 0.1 ng per tube, 50% inhibition of binding occurred with 12.8 +/- 1.2 ng of unlabeled ovine prolactin standar...

The twin arginine translocation (Tat) system moves folded proteins across the cytoplasmic membrane of bacteria and the thylakoid membrane of plant chloroplasts. Signal peptide-bearing substrates of the Tat pathway (precursor proteins) are recognized at the membrane by the TatBC receptor complex. The only established preparation of the TatBC complex uses the detergent digitonin, rendering it uns...

Potentiometric membrane electrodes that respond to heparin and other polyanions were introduced in the early 1990s. Herein, the mechanism of polymer membrane electrode type heparin sensors is revisited. The extraction/diffusion of heparin is studied via both potentiometric and impedance spectroscopic techniques using a pre-fractionated heparin preparation that contains polyanionic species > 100...