Type 1 spinal muscular atrophy (SMA) is a progressive neuromuscular disease characterized by an onset at 6 months of age or younger, inability to sit without support, and deficient levels survival motor neuron (SMN) protein. Risdiplam orally administered small molecule that modifies SMN2 pre–messenger RNA splicing increases functional SMN protein in blood.

The major mRNA for adenovirus 2 polypeptide pVIII sediments at 18S as assayed by in vitro translation in the messenger-dependent rabbit reticulocyte lysate system. However, a small amount of messenger activity for pVIII sediments at abut 27S, coincident with the mRNA for 100 K. Isolation and fractionation of poly(A) containing RNA following in vitro translation of 27S 100 K mRNA demonstrated th...

The messenger activity for fructose 1,6-bisphosphate aldolase (EC4.1.2.13) (aldolase) A isozyme has been characterized in the polysome- or the messenger RNA-directed, protein-synthesizing system using the pH 5 fraction of rat liver or wheat germ extracts, respectively. The subunit of aldolase A synthesized in vitro was detected by immunoprecipitation with anti-aldolase A antibody raised in chic...

This chapter provides a review of recent advances in understanding the importance of alternative pre-messenger RNA splicing in stem cell biology. The majority of transcribed pre-mRNAs undergo RNA splicing where introns are excised and exons are juxtaposed to form mature messenger RNA sequences. This regulated, selective removal of whole or portions of exons by alternative splicing provides aven...

In eukaryotes, messenger RNA biogenesis depends on the ordered and precise assembly of a nuclear messenger ribonucleoprotein particle (mRNP) during transcription. This process requires a well-orchestrated and dynamic sequence of molecular recognition events by specific RNA-binding proteins. Arginine methylation is a posttranslational modification found in a plethora of RNA-binding proteins resp...

the genome of iranian wheat stripe virus (iwsv), a tentative member of the genus tenuivirus, is comprised of three ambisense and one negative sense rna segments. the coat and non-structural proteins encoded by the vcrna3 and vrna4 genes, respectively, were efficiently translated in vitro. translated proteins of vcrna3 and vrna4 transcripts were approximately 35000 and 22000 in mr, respectively,...

tmrna (also known as ssra or 10sa rna) is so-named for its dual nature so that can function like trna and mrna. tmrna involved in a trans-translation reaction that truly is a kind of translational quality control system. this process contributes to the recycling of stalled translation complexes at the end of an mrna lacking a stop codon or at an internal mrna cluster of rare codons in a process...

We consider a system of coupled reaction-diffusion equations that models the interaction between multiple types of chemical species, particularly the interaction between one messenger RNA and different types of non-coding microRNAs in biological cells. We construct various modeling systems with different levels of complexity for the reaction, nonlinear diffusion, and coupled reaction and diffus...

RNA was first explored and understood as a messenger molecule, relaying DNA encodings of amino acids during protein synthesis. Beyond messenger RNA, however, a class of RNA known as non-coding RNA plays fundamental roles in transcriptional and translational gene regulation. As the biological mantra goes, form fits function, and these regulatory roles depend on the 3-D structure of the RNA molec...