Anaerobic methane oxidation coupled to denitrification was recently assigned to bacteria belonging to the uncultured phylum NC10. In this study, we incubated sediment from a eutrophic ditch harboring a diverse community of NC10 bacteria in a bioreactor with a constant supply of methane and nitrite. After 6 months, fluorescence in situ hybridization showed that NC10 bacteria dominated the result...

The abundances and activities of aerobic methane-oxidizing bacteria (MOB) were compared in depth profiles of littoral and profundal sediments of Lake Constance, Germany. Abundances were determined by quantitative PCR (qPCR) targeting the pmoA gene and by fluorescence in situ hybridization (FISH), and data were compared to methane oxidation rates calculated from high-resolution concentration pro...

The integration of closed biotechnological cycles in life support systems (LSS) autonomous transport (ATS) is a critical solution for deep space and planetary exploration. Prolonged existence integral ecosystems depends on the degree cyclic use substances coefficient closure ecosystem's mass-exchange processes. In missions lasting over two years, it becomes more beneficial to reproduce necessar...

e role of the microbial processes governing methane emissions from tundra ecosystem is receiving increasing attention. Recently, cooperation between methanotrophic bacteria and submerged Sphagnum was shown to reduce methane emissions but also to supply CO2 for photosynthesis for the plant. Although this process was shown to be important in the laboratory, the differences that exist in methane ...

The importance of anaerobic oxidation of methane (AOM) as a methane sink in freshwater systems is largely unexplored, particularly in peat ecosystems. Nitrite-dependent anaerobic methane oxidation (n-damo) was recently discovered and reported to be catalyzed by the bacterium "Candidatus Methylomirabilis oxyfera," which is affiliated with the NC10 phylum. So far, several "Ca. Methylomirabilis ox...

Microbial communities of methanogenic, sulfate-reducing and methanotrophic prokaryotes from deepsea environments were investigated by molecular phylogenetic analysis of the deduced amino acid sequences of the genes encoding for the methyl coenzyme M reductase (mcrA), dissimilatory sulfite reductase (dsrAB) and particulate methane monoxygenase (pmoA), respectively. Clone libraries of PCR amplifi...

In stratified lakes, methane oxidizing bacteria are critical converters that significantly reduce emissions of this greenhouse gas to the atmosphere. Efforts better understand their ecology uncovered a surprising diversity, vertical structure, and seasonal succession. It is an open question how diversity has be considered in models microbial oxidation. Likewise, it unclear what extent simple tr...

The geochemistry of rare earth elements and yttrium (REY) in phosphorite has been widely studied. However, the effect organic matter on REY enrichment not well determined. We utilized paired inorganic (δ13Ccarb) carbon isotopes, total (TOC), content (∑REY) Zhijin Motianchong (MTC) compared them with those Meishucun (MSC) to reveal REY. δ13Ccarb MTC area (≈0‰) is heavier than that MSC (−5.23‰ −1...