The basis of enzyme kinetic modelling was established during the early 1900’s when the work of Leonor Michaelis and Maud Menten produced a pseudo-steady state equation linking enzymatic catalytic rate to substrate concentration (Michaelis & Menten, 1913). Building from the Michaelis-Menten equation, other equations used to describe the effects of modifiers of enzymatic activity were developed b...

A simple mathematical model of steady state  oxygen distribution subject to diffusive transport and non- linear uptake in a retinal cylinder has been developed. The approximate analytical solution to a reaction- diffusion equation are obtained by using series expansions. The computational results for the scaled variables are presented through graphs. The effect of the important parameters (1) d...

The unidirectional influx of cycloleucine into in vitro preparations of gill tissue of the mussel, Mytilus californianus, was determined. Influx was found to be linear for at least an hour, and the kinetics of cycloleucine influx conformed to Michaelis-Menten type kinetics. The transport mechanism(s) for cycloleucine is relatively specific for the L-enantiomorph of neutral amino acids, and is c...

Two forms of glutathione S-aryltransferase were purified from rat liver. The only differences noted between the two forms were in the chromatographic and electrophoretic properties, which permitted the separation of the two species. The molecular weights of the enzyme and its subunits were estimated as about 50000 and 23000 respectively. The steady-state kinetics did no follow Michaelis-Menten ...

Abstract The venerable process of cellular respiration is essential for cells to produce energy from glucose molecules, in order carry out work. responsible producing molecules ATP, a molecule which thermodynamically coupled with other biochemical and biophysical processes provide such occur. While the biology, one cycle occurs only matter milliseconds, so, it would be impractical measure time ...

In most of the real life problems, we encounter with nonlinear differential equations. Problems are made more understandable by modeling them these this way, it becomes easier to interpret problems and reach results. 1913, basic enzymatic reaction model introduced Michaelis Menten describe enzyme processes is an example equation. This one simplest best-known approaches mechanisms used enzyme-ca...

catalytic isomerization of glucose to fructose by immobilized glucose isomerase in an aqueous-ethanol medium was studied. using michaelis-menten equation and haldane relationship, the main kinetic parameters were determined: kmf, vmf, kmr, vmr. by comparing the aqueous-ethanol medium with an aqueous solution, the measured values of kmr and kmf were increased in the aqueous-ethanol solution, whi...

Basimglurant, a novel mGlu5-negative allosteric modulator under development for the treatment of major depressive disorder, is cleared via cytochrome P450 (P450)-mediated oxidative metabolism. Initial enzyme phenotyping studies indicated that CYP3A4/5 dominates basimglurant metabolism and highlights a risk for drug-drug interactions when it is comedicated with strong CYP3A4/5 inhibitors or inac...