Dental pulp cells (DPCs) are promising candidates for use as transplantable cells in regenerative medicine. However, ex vivo expansion of these cells typically requires culture media containing fetal bovine serum, which may cause infection and immunological reaction following transplantation. In addition, the proliferation and differentiation of DPCs markedly depend upon serum batches. Therefor...

Fetal bovine serum (FBS) has been reported to affect chondrocyte biosynthesis in monolayer culture. Insulin-Transferrin-Selenium (ITS) was investigated as a partial replacement for FBS during in vitro culture of rabbit articular chondrocytes in three-dimensional alginate scaffold. Chondrocyte-seeded alginate hydrogels were cultured in Dulbecco's modified Eagle's medium plus 10% FBS, 1% ITS plus...

The ability to vary systematically the neuronal environment is one advantage afforded by the use of cell culture. Replacement of serum, a variable and undefined medium supplement, with known ingredients allows even greater control of culture conditions. We have studied biochemical and morphological properties related to neurotransmitter metabolism of rat sympathetic neurons cultured in a modifi...

The open pulled straw has been used effectively to vitrify preimplantation embryos because of geometricfeatures that allow rapid rates of temperature exchange. One possible inexpensive alternative to the openpulled straw are commercially-available microcapillary pipette tips commonly used for electrophoresis. Themain purpose of this study was to compare the survival rates of in vitro produced b...

background: there is a growing demand for mass production of shikalkin (a natural pigment consisted of shikonin and alkannin) due to its increasing applications in cosmetics, pharmaceutical and nutrition industries. the root of iranian arnebia euchroma produces shikalkin. the promising capability of this plant for shikalkin production has already been demonstrated in cell culture studies. objec...

embryonic stem cells (escs) are derived from the inner cell mass (icm) of blastocyst and differentiate into all three embryonic germ layers: ectoderm, endoderm, and mesoderm. in this study, escs are derived from hand made cloning (hmg) blastocysts and their efficiencies compared to escs derived from in vitro fertilization (ivf) embryos. feeder layer was used for escs culture, and culture medium...

LEVEL: INTERMEDIATE M onoclonal antibodies are used to treat a wide range of illnesses such as rheumatoid arthritis, Crohn’s Disease, and follicular non-Hodgkin lymphoma (1). The identification and use of certain growth medium additives and modified culture conditions during cell culture processes that produce MAbs may lower production costs—which could help lower the overall costs of those dru...

Ruminal ciliates have been grown in continuous culture in chemically defined media and in the absence of viable bacteria. Oligotrichic ruminal ciliates seem to require insoluble carbohydrates for growth; the holotrichic ciliates require soluble carbohydrates, but at low concentrations. Both groups of ciliates utilize amino acids as their principal nitrogen source when these are supplied in micr...

BACKGROUND Traditionally, the Lowenstein Jensen (LJ) medium has been used for culturing Mycobacterium tuberculosis. In abdominal tuberculosis (TB), the reported yield from tissue culture is between 20% and 60%. Liquid cultures are reported to give a higher yield but there is little data available in abdominal TB. AIM To compare the yield of TB culture with BACTEC 460TB liquid medium and LJ me...

Despite recent advances, tuberculosis (TB) diagnosis remains imperfect in resource-limited settings due to its complexity and costs, poor sensitivity of available tests, or long times to reporting. We present a report on the use of colorimetric methods, based on the detection of mycobacterial growth using colorimetric indicators, for the detection of Mycobacterium tuberculosis in sputum specime...