Gibson Assembly® cloning has proven to be useful as a molecular biology technique for the seamless assembly of synthetic and natural genes and large-scale genetic pathways. Notably, Gibson Assembly cloning has enabled the synthesis of the first bacterial genome1, the first synthetic cell2, and the first minimal cell3. Additionally, the Gibson Assembly method has been utilized for genetic recodi...

genetic diversity among plants sprouted from seven year old clones of mulberry (morus indica var. s36) was studied using molecular markers such as issr and rapd. the clones showed significant variability in sex expression and the sprouting behavior. these changes were appeared after seven years of rigorous pruning and training (pruned once in every 3-4months). genetic diversity analysis reveale...