نتایج جستجو برای: monolayer culture
تعداد نتایج: 295239 فیلتر نتایج به سال:
Recent studies have demonstrated that orally administered thiazolidinedione ligands of the peroxisome proliferator-activated receptor-gamma can ameliorate clinical features of psoriasis in humans. Thiazolidinediones also inhibit the proliferation of psoriatic keratinocytes in monolayer and organ culture, and at least one of these agents (troglitazone) inhibits epidermal hyperplasia of human pso...
ZR-75-1 human mammary cancer cells can be grown in serumfree medium in an estradiciand tamoxifen-sensitive manner. Growth occurs both in monolayer and suspension culture, al though serum is necessary during the initial plating of the cells in monolayer culture. Optimal stimulation of proliferation by estradiol occurs at 10~8 and 10~9 M. Tamoxifen alone weakly stimulated growth in a dose-depende...
Several protocols have been developed for human induced pluripotent stem cell neuronal differentiation. We compare several methods for forebrain cortical neuronal differentiation by assessing cell morphology, immunostaining and gene expression. We evaluate embryoid aggregate vs. monolayer with dual SMAD inhibition differentiation protocols, manual vs. AggreWell aggregate formation, plating subs...
Hepatocyte function on 3-D microfabricated polymer scaffolds realised with the pressure-activated microsyringe was tested under static and dynamic conditions. The dynamic cell culture was obtained using the multicompartment modular bioreactor system. Hepatocyte cell density, glucose consumption, and albumin secretion rate were measured daily over a week. Cells seeded on scaffolds showed an incr...
A single wave of mitotic activity was observed in a monolayer culture of rat keratinocytes immediately after exposure to 12-O-tetradecanoylphorbol-13-acetate. A peak for cells in prophase, observed at 10 min after the exposure, was followed by a peak for metaphase at 20 min, for anaphase at 25 min and telophase at 30 min after the exposure. Thereafter, the mitotic activity began to subside. Thi...
Rabbit whole meibomian glands were isolated by microsurgical dissection and enzymatic digestion. Both ductal and acinar elements could be separated by subsequent microsurgical manipulation. Epithelial outgrowth could be derived from either the whole gland or the ductal/acinar elements using collagen gel in the serum-containing, growth factor-enriched medium. Two types of outgrowths were observe...
Three-dimensional (3D) cultures are increasing in use because of their ability to represent in vivo human physiology when compared to monolayer two-dimensional (2D) cultures. When grown in 3D using scaffold-free agarose hydrogels, MCF-7 human breast cancer cells self-organize to form directionally-oriented microtissues that contain a luminal space, reminiscent of the in vivo structure of the ma...
BACKGROUND Mesenchymal stem cells (MSCs) are of great interest in bone regenerative medicine due to their osteogenic potential and trophic effects. However, challenges to large-scale production of MSCs can hinder the translation of MSC therapies. 3D Microcarrier (MC)-based MSC culture presents a scalable and cost-effective alternative to conventional methods of expansion in 2D monolayers. Furth...
Cell-based therapy has been explored for articular cartilage regeneration. Autologous chondrocyte implantation is a promising cell-based technique for repairing articular cartilage defects. However, there are several issues such as chondrocyte de-differentiation. While numerous studies have been designed to overcome some of these issues, only a few have focused on the thermal environment that c...
The dependence of the phagocytosis of particulate materials on their size was studied. Rabbit alveolar macrophages (AMs) obtained by lung lavage were cultured in suspensions or monolayers with Latex particles of 1 micron or 2 microns in diameter. After culturing AMs with 10(7)(-5) X 10(9) Latex particles per ml for 15 to 360 minutes, the number of phagocytized particles in each of 100 individua...
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