A new concept for the development of semiconductor field-effect biosensors by modification of a gate surface with tobacco mosaic virus (TMV) nanotubes, serving as enzyme nanocarrier, is presented. TMV nanotubes enable an immobilization of a high amount of enzymes without substantial loss of their activity, resulting in an enhanced biosensor performance. This approach has been experimentally dem...

In Nicotiana glutinosa L. formation of local lesions on lower leaves inoculated with tobacco mosaic virus increased the susceptibility of the upper leaves to infection in a subsequent inoculation. The increase in susceptibility was detected as an increase of up to 3"5-fold in the number of lesions produced on the upper leaf and a corresponding increase in the amount of virus RNA synthesized. Th...

The RNA replicase extracted from Brome mosaic virus (BMV)-infected plants has been used to characterize the cis-acting elements for RNA synthesis and the mechanism of RNA synthesis. Minus-strand RNA synthesis in vitro requires a structure named stem-loop C (SLC) that contains a clamped adenine motif. In vitro, there are several specific requirements for SLC recognition. We examined whether thes...

Professor, Department of Plant Pathology Cornell University Ithaca, New York 14853 May, 2001 Abstract Barley yellow dwarf virus (BYDV) and Cereal yellow dwarf virus (CYDV) are two aphidvectored, viral disease which most typically strikes small grains, especially barley, oats, and wheat. Limited research has been conducted regarding its reaction in sweet corn because BYDV/CYDV have not previousl...

Begomoviruses (Geminiviridae) are serious plant pathogens infecting dicotyledonous plants among important crop plants. The majority of the members comprises bipartite genomes (DNA A and B), encapsidated in twin particles and transmitted by the whitefly-vector Bemisia tabaci. Cassava (Manihot esculenta, Crantz) production is restricted by begomovirus inducing Cassava mosaic disease (CMD) in Afri...

Deletions on the long-arm of chromosome 20, del(20q), are common karyotypic abnormalities in myeloid disorders. Bioinformatic analyses of the B-allele frequency and log R ratio values from genome-wide association data have identified individuals who are mosaic for large structural abnormalities (>2 Mb). We investigated the most common autosomal event, namely mosaic del(20q), in 46 254 nonhemato...

Almost the whole of our evidence for the internal structure of the particles is derived from x-ray observations, particularly those of the high angle or intramolecular photographs. In contradistinction to the low angle photographs, the most striking feature is that the general appearance of the pattern is independent of the state of hydration. Most of the photographs have been taken from orient...

The nucleotide sequences of the coat protein genes and 3' non-coding regions of two aphid-transmissible isolates (G2 and G7) of soybean mosaic virus (SMV) were determined. The coat protein of the G2 isolate differs from that of the aphid non-transmissible N isolate by a single amino acid at position 12 (aspartic acid in N, glycine in G2 and G7). The G7 isolate differs from G2 and N at three and...

Clones have been constructed that contain full-length cDNA copies of cowpea mosaic virus RNA1 and RNA2, downstream of the cauliflower mosaic virus 35S promoter. The clones, when linearized downstream of the viral sequences, give rise to cowpea mosaic virus-like symptoms when inoculated onto cowpea plants. Viral RNA and virions can be detected in the inoculated plants, demonstrating that the clo...